Literature summary for 1.1.1.81 extracted from

Kleczkowski, L.A.; Edwards, G.E.; Blackwell, R.D.; Lea, P.J.; Givan, C.V.
Enzymology of the reduction of hydroxypyruvate and glyoxylate in a mutant of barley lacking peroxisomal hydroxypyruvate reductase (1990), Plant Physiol., 94, 819-825.

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Inhibitors

Inhibitors	Comment	Organism	Structure
Hydroxypyruvate	2 mM, 79% inhibition of enzyme activity in LaPr 88/29 mutant which lacks NADH-prefering hydroxypyruvate reductase	Hordeum vulgare
Tartronate	2 mM, 83% inhibition of enzyme activity in LaPr 88/29 mutant which lacks NADH-prefering hydroxypyruvate reductase	Hordeum vulgare

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.08	-	Hydroxypyruvate	enzyme activity of wild-type in 40-60% precipitate fractions after ammonium sulfate fractionation	Hordeum vulgare
0.71	-	Hydroxypyruvate	enzyme activity of LaPr 88/29 mutant which lacks NADH-prefering hydroxypyruvate reductase in 40-60% precipitate fractions after ammonium sulfate fractionation	Hordeum vulgare

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
hydroxypyruvate + NAD(P)H	Hordeum vulgare	-	D-glycerate + NAD(P)+ + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Hordeum vulgare	-	barley	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Hordeum vulgare	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.054	-	enzyme activity in leaf extracts of LaPr 88/29 mutant which lacks NADH-prefering hydroxypyruvate reductase	Hordeum vulgare
0.068	-	enzyme activity in leaf extracts of wild type	Hordeum vulgare

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hydroxypyruvate + NAD(P)H	-	Hordeum vulgare	D-glycerate + NAD(P)+ + H+	-	?

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Release 2023.1 (January 2023)