Cloned (Comment) | Organism |
---|---|
expressed in a gpd1DELTAgpd2DELTA double deletion mutant of Saccharomyces cerevisiae | Wickerhamiella versatilis |
expressed in Saccharomyces cerevisiae strain BY4741 | Wickerhamiella versatilis |
gene Cagpd1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval. Recombinant expression in the Sacchhyromyces cerevisiae strain BY4741 gpd1DELTAgpd2DELTA double mutant | Wickerhamiella versatilis |
gene Cagpd2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval. Recombinant expression in the Sacchhyromyces cerevisiae strain BY4741 gpd1DELTAgpd2DELTA double mutant | Wickerhamiella versatilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Wickerhamiella versatilis | - |
glycerone phosphate + NADH + H+ | - |
? | |
sn-glycerol 3-phosphate + NAD+ | Wickerhamiella versatilis | - |
glycerone phosphate + NADH + H+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | Wickerhamiella versatilis SN-18 | - |
glycerone phosphate + NADH + H+ | - |
? | |
sn-glycerol 3-phosphate + NAD+ | Wickerhamiella versatilis SN-18 | - |
glycerone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Wickerhamiella versatilis | A0A140JW76 | - |
- |
Wickerhamiella versatilis | A0A140JW76 | an osmotolerant yeast isolated from soil outside a food factory | - |
Wickerhamiella versatilis | A0A140JW77 | - |
- |
Wickerhamiella versatilis | A0A140JW77 | an osmotolerant yeast isolated from soil outside a food factory | - |
Wickerhamiella versatilis SN-18 | A0A140JW76 | - |
- |
Wickerhamiella versatilis SN-18 | A0A140JW76 | an osmotolerant yeast isolated from soil outside a food factory | - |
Wickerhamiella versatilis SN-18 | A0A140JW77 | - |
- |
Wickerhamiella versatilis SN-18 | A0A140JW77 | an osmotolerant yeast isolated from soil outside a food factory | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0009 | - |
crude enzyme extract, pH 8.5, 30°C | Wickerhamiella versatilis |
0.00147 | - |
crude enzyme extract, pH 8.5, 30°C | Wickerhamiella versatilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with NADP+ | Wickerhamiella versatilis | ? | - |
? | |
additional information | no activity with NADP+ | Wickerhamiella versatilis SN-18 | ? | - |
? | |
sn-glycerol 3-phosphate + NAD+ | - |
Wickerhamiella versatilis | glycerone phosphate + NADH + H+ | - |
? | |
sn-glycerol 3-phosphate + NAD+ | - |
Wickerhamiella versatilis | glycerone phosphate + NADH + H+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | - |
Wickerhamiella versatilis SN-18 | glycerone phosphate + NADH + H+ | - |
? | |
sn-glycerol 3-phosphate + NAD+ | - |
Wickerhamiella versatilis SN-18 | glycerone phosphate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Cagpd1 | - |
Wickerhamiella versatilis |
Cagpd1 | isoform | Wickerhamiella versatilis |
Cagpd1p | - |
Wickerhamiella versatilis |
Cagpd2 | - |
Wickerhamiella versatilis |
Cagpd2 | isoform | Wickerhamiella versatilis |
Cagpd2p | - |
Wickerhamiella versatilis |
glycerol 3-phosphate dehydrogenase | - |
Wickerhamiella versatilis |
GPD | - |
Wickerhamiella versatilis |
Gpd1p | - |
Wickerhamiella versatilis |
gpd2p | - |
Wickerhamiella versatilis |
NADP+-dependent glycerol 3-phosphate dehydrogenase | - |
Wickerhamiella versatilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Wickerhamiella versatilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Wickerhamiella versatilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | Cagpd1p is a NAD+-dependent GPD, no activity with NADP+ | Wickerhamiella versatilis | |
additional information | Cagpd2p is a NAD+-dependent GPD, no activity with NADP+ | Wickerhamiella versatilis | |
NAD+ | - |
Wickerhamiella versatilis | |
NADH | - |
Wickerhamiella versatilis |
Organism | Comment | Expression |
---|---|---|
Wickerhamiella versatilis | osmotic stress induces the isozyme | up |
General Information | Comment | Organism |
---|---|---|
evolution | the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval | Wickerhamiella versatilis |
metabolism | the enzyme is a key enzyme in the glycerol biosynthesis pathway | Wickerhamiella versatilis |
physiological function | the enzyme is involved in osmotic stress response | Wickerhamiella versatilis |
physiological function | two glycerol 3-phosphate dehydrogenase isozymes from Candida versatilis SN-18 play an important role in glycerol biosynthesis under osmotic stress. The enzyme is involved in osmotic stress response | Wickerhamiella versatilis |