Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dihydroxyacetone phosphate | 0.5 mM, GPDHs more susceptible to inhibition | Drosophila virilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values of two further allelic isozymes, alpha-GPDHf and alpha-GPDHm | Drosophila virilis | |
0.00526 | - |
NADH | alpha-GPDHs | Drosophila virilis | |
0.1003 | - |
NAD+ | alpha-GPDHs, standard deviation 0.0041 mM | Drosophila virilis | |
0.2144 | - |
dihydroxyacetone phosphate | alpha-GPDHs, standard deviation 0.0215 mM | Drosophila virilis | |
1.89 | - |
glycerol 3-phosphate | alpha-GPDHs, standard deviation 0.25 mM | Drosophila virilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
gel filtration | Drosophila virilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Drosophila virilis | - |
dihydroxyacetone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila virilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
3 allelic forms distinguishable by electrophoresis | Drosophila virilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
71.8 | - |
- |
Drosophila virilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | - |
Drosophila virilis | dihydroxyacetone phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000-37000, SDS-PAGE | Drosophila virilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
isozyme GPDHf, activity falls off at temperature above 30°C | Drosophila virilis |
40 | - |
isozyme GPDHm and GPDHs | Drosophila virilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
most stable at pH 8, activity reduced, GPDHf to a greater extent | Drosophila virilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | maximal activities of the three allelic forms are obtained within a pH range of 6.0-6.5 for dihydroxyacetone phosphate reduction | Drosophila virilis |
10 | 10.5 | maximal activities of the three allelic forms are obtained within a pH range of 10.0-10.5 for glycerol 3-phosphate oxidation | Drosophila virilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
rate of inactivation increases below pH 7 | Drosophila virilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Drosophila virilis | |
NADH | - |
Drosophila virilis |