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Literature summary for 1.1.1.76 extracted from

  • Ui, S.; Takusagawa, Y.; Ohtsuki, T.; Mimura, A.; Ohkuma, M.; Kudo, T.
    Stereochemical applications of the expression of the L-2,3-butanediol dehydrogenase gene in Escherichia coli (2001), Lett. Appl. Microbiol., 32, 93-98.
    View publication on PubMed
Search for more literature for 1.1.1.76

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli JM109, stereospecificity of resulting L-butanediol dehydrogenase is reduced. Expressed gene contains tac-promoter and upstream region of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae IAM1063. A more stable stereospecificity is exhibited Corynebacterium glutamicum
When the enzyme gene is only expressed in Escherichia coli using the tac promoter, the stereospecificity of resulting enzyme gene is reduced. When the region upstream of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae is also involved in the expression of gene, the resulting enzyme exhibits more stable stereospecificity. Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
 C-1012
-
Corynebacterium glutamicum C-1012
-
 C-1012
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 highest specific activities without glucose addition to culture medium, induction with 0.25 mg/ml isopropyl-beta-thiogalactopyranosid Corynebacterium glutamicum
1.51
-
 plasmid containing L-butanediol dehydrogenase gene Corynebacterium glutamicum
17
-
 plasmid containing tac promoter and L-butanediol dehydrogenase ORF Corynebacterium glutamicum
18.6
-
 plasmid containing meso-butanediol dehydrogenase promoter region and L-butanediol dehydrogenase ORF Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-acetoin + NADH short chain dehydrogenase reductase family Corynebacterium glutamicum L-2,3-butanediol
-
 r
L-acetoin + NADH exhibits marked sequence similarity and common functionally conserved sequence with meso-enzyme Corynebacterium glutamicum L-2,3-butanediol
-
 r
L-acetoin + NADH short chain dehydrogenase reductase family Corynebacterium glutamicum C-1012 L-2,3-butanediol
-
 r
L-acetoin + NADH exhibits marked sequence similarity and common functionally conserved sequence with meso-enzyme Corynebacterium glutamicum C-1012 L-2,3-butanediol
-
 r
L-acetoin + NADH + H+ To confirm the high production of enzyme, the conversion of L-acetoin, in a racemic mixture, to L-2,3-butanediol is studied. 0.37% L-2,3-butanediol is formed from 1% L-acetoin added to the culture. Corynebacterium glutamicum (S,S)-butanediol + NAD+
-
 ?
L-acetoin + NADH + H+ To confirm the high production of enzyme, the conversion of L-acetoin, in a racemic mixture, to L-2,3-butanediol is studied. 0.37% L-2,3-butanediol is formed from 1% L-acetoin added to the culture. Corynebacterium glutamicum C-1012 (S,S)-butanediol + NAD+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Corynebacterium glutamicum
NADH
-
 Corynebacterium glutamicum