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Literature summary for 1.1.1.75 extracted from
- Threonine metabolism in a strain of Bacillus subtilis enzymic oxidation of 1-aminopropan-2-ol and aminoacetone (1971), Biochim. Biophys. Acta, 252, 98-104.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 31 | - |
L-1-Aminopropan-2-ol | - |
Bacillus subtilis |  |
| 54 | - |
D-1-aminopropan-2-ol | - |
Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | stimulating at all concentrations | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 250000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-1-aminopropan-2-ol + NAD+ | Bacillus subtilis | - |
aminoacetone + NADH | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.001 | - |
- |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, 0.1 M Tris/HCl buffer, pH 7.2, 50% loss of activity | Bacillus subtilis |
| 0°C, 0.1 M Tris/HCl buffer, pH 7.2, 90% loss of activity | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-diaminopropan-2-ol + NAD+ | - |
Bacillus subtilis | 2-aminoacetamide + NADH | - |
? | |
| 1-amino-3-diethylaminopropan-2-ol + NAD+ | - |
Bacillus subtilis | 2-amino-N,N-diethylacetamide + NADH | - |
? | |
| 1-aminopropan-2,3-diol + NAD+ | - |
Bacillus subtilis | ? | - |
? | |
| butan-2,3-diol + NAD+ | - |
Bacillus subtilis | ? | - |
? | |
| D,L-1-aminopropan-2-ol + NAD+ | D-enantiomer 50% of activity with L-enantiomer | Bacillus subtilis | aminoacetone + NADH | - |
ir | |
| D-1-aminopropan-2-ol + NAD+ | - |
Bacillus subtilis | aminoacetone + NADH + H+ | - |
r | |
| hydroxyacetone + NAD+ | no activity with acetone | Bacillus subtilis | 2-oxopropanal + NADH | - |
? | |
| L-1-aminopropan-2-ol + NAD+ | - |
Bacillus subtilis | aminoacetone + NADH | - |
ir | |
| propan-1,2-diol + NAD+ | - |
Bacillus subtilis | ? | - |
? | |
| propan-1,3-diol + NAD+ | - |
Bacillus subtilis | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | - |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
sharp optimum | Bacillus subtilis |
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