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Literature summary for 1.1.1.67 extracted from

  • Puttick, J.; Vieille, C.; Song, S.H.; Fodje, M.N.; Grochulski, P.; Delbaere, L.T.
    Crystallization, preliminary X-ray diffraction and structure analysis of Thermotoga maritima mannitol dehydrogenase (2007), Acta Crystallogr. Sect. F, 63, 350-352.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene mtdh, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme, 10 mg/ml protein in 50 mM Tris-HCl, pH 8.5, room temperature, microbatch-under-oil method, mixing of equal volumes of protein and crystallization solutions, the latter containing 30% 2-methyl-2,4-pentanediol, and 0.1 M Na HEPES, pH 7.5, X-ray diffraction structure determination and analysis at 3.3 A resolution, molecular-replacement Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
gene mtdh
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Thermotoga maritima

Synonyms

Synonyms Comment Organism
mannitol dehydrogenase
-
Thermotoga maritima
MtDH
-
Thermotoga maritima