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Literature summary for 1.1.1.62 extracted from
- Purification and characterization of epimeric estradiol dehydrogenases (17alpha and 17beta) from equine placenta (1984), Biochemistry, 23, 486-491.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
estradiol-17beta | - |
Equus caballus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Equus caballus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
2 * 33000, SDS-PAGE | Equus caballus |
| 68500 | - |
gel filtration | Equus caballus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Equus caballus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Equus caballus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Equus caballus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.7 | - |
- |
Equus caballus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, phosphate buffer, pH 7.0, 1 mM dithioerythritol, stable for at least 1 month | Equus caballus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| estradiol-17beta + NAD+ | enzyme prefers NAD+ over NADP+ | Equus caballus | estrone + NADH | - |
? | |
| estrone + NADH | enzyme prefers NAD+ over NADP+ | Equus caballus | estradiol-17beta + NAD+ | - |
? | |
| estrone + NADPH + H+ | - |
Equus caballus | estradiol-17beta + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 33000, SDS-PAGE | Equus caballus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.5 | - |
Equus caballus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 10 | about 60% of maximal activity at pH 8.5 and at pH 10.0 | Equus caballus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | cofactor | Equus caballus | |
| NAD+ | enzyme prefers NAD+ over NADP+ | Equus caballus | |
| NADP+ | enzyme prefers NAD+ over NADP+ | Equus caballus | |
| NADPH | cofactor | Equus caballus | |
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