Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3(3-dimethylaminopropyl)carbodiimide | - |
Cellulomonas sp. | |
2,4,6-Trinitrobenzene sulfonate | - |
Cellulomonas sp. | |
acetone | inactivation, enzyme regains activity after removal of ketone | Cellulomonas sp. | |
Anhitol 24B | - |
Cellulomonas sp. | |
catechol | i.e. 1,2-butanediol, inactivation, enzyme regains activity after removal of alcohol | Cellulomonas sp. | |
Cetylpyridinium chloride | strong inhibition at low concentration | Cellulomonas sp. | |
cetyltrimethylammonium bromide | strong inhibition at low concentration | Cellulomonas sp. | |
D-xylose | - |
Cellulomonas sp. | |
diacetyl | inactivation, enzyme regains activity after removal of ketone | Cellulomonas sp. | |
dimethylformamide | inactivation | Cellulomonas sp. | |
Dimethylsulfoxide | inactivation | Cellulomonas sp. | |
dioxane | inactivation, enzyme regains activity after removal of ketone | Cellulomonas sp. | |
DTNB | - |
Cellulomonas sp. | |
ethanol | 10% v/v: 25-75% loss of activity, depending on substrate, enzyme regains activity after removal of alcohol | Cellulomonas sp. | |
iodoacetate | - |
Cellulomonas sp. | |
isobutanol | 5% v/v: 0-50% loss of activity, depending on substrate, enzyme regains activity after removal of alcohol | Cellulomonas sp. | |
meso-erythritol | - |
Cellulomonas sp. | |
methanol | 10% v/v: 25-75% loss of activity, 5% v/v, 50-95% loss of activity, depending on substrate, enzyme regains activity after removal of alcohol | Cellulomonas sp. | |
methylene blue | enzyme activity is decreased by photooxidation | Cellulomonas sp. | |
n-amyl alcohol | 2% v/v: 0-50% loss of activity, depending on substrate, enzyme regains activity after removal of alcohol | Cellulomonas sp. | |
n-butanol | 5% v/v: 0-50% loss of activity, depending on substrate, enzyme regains activity after removal of alcohol | Cellulomonas sp. | |
n-Propanol | 5% v/v: 25-60% loss of activity, depending on substrate, enzyme regains activity after removal of alcohol | Cellulomonas sp. | |
PCMB | - |
Cellulomonas sp. | |
Sodium dodecyl sulfate | - |
Cellulomonas sp. | |
sucrose | - |
Cellulomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0238 | - |
2,3-Butanediol | - |
Cellulomonas sp. | |
0.202 | - |
1,2-propanediol | - |
Cellulomonas sp. | |
10.9 | - |
glycerol | - |
Cellulomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | soluble, isolation from crude cell extract | Cellulomonas sp. | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | Cellulomonas sp. | - |
dihydroxyacetone + NADH + H+ | - |
? | |
glycerol + NAD+ | Cellulomonas sp. NT3060 | - |
dihydroxyacetone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas sp. | - |
- |
- |
Cellulomonas sp. NT3060 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cellulomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol + NAD+ | - |
Cellulomonas sp. | hydroxyacetone + NADH | - |
? | |
1,2-propanediol + NAD+ | - |
Cellulomonas sp. NT3060 | hydroxyacetone + NADH | - |
? | |
2,3-butanediol + NAD+ | - |
Cellulomonas sp. | 3-hydroxybutane-2-one + NADH | - |
? | |
2,3-butanediol + NAD+ | - |
Cellulomonas sp. NT3060 | 3-hydroxybutane-2-one + NADH | - |
? | |
glycerol + NAD+ | - |
Cellulomonas sp. | dihydroxyacetone + NADH + H+ | - |
? | |
glycerol + NAD+ | - |
Cellulomonas sp. NT3060 | dihydroxyacetone + NADH + H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cellulomonas sp. | |
NADH | - |
Cellulomonas sp. |