Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | - |
Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
NADH | - |
Klebsiella aerogenes | |
0.15 | - |
NAD+ | - |
Klebsiella aerogenes | |
1.3 | - |
dihydroxyacetone | - |
Klebsiella aerogenes | |
39 | - |
glycerol | - |
Klebsiella aerogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | soluble, isolation from crude cell extract | Klebsiella aerogenes | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | Klebsiella aerogenes | - |
dihydroxyacetone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
82 | - |
- |
Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
frozen, retains about 42% of initial activity for 2 months | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol + NAD+ | as active as glycerol | Klebsiella aerogenes | hydroxyacetone + NADH | - |
? | |
1,3-propanediol + NAD+ | relative rate of oxidation is 37% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
1,4-butanediol + NAD+ | relative activity 17% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
2,3-butanediol + NAD+ | as active as glycerol | Klebsiella aerogenes | 3-hydroxybutane-2-one + NADH | - |
? | |
beta-glycerophosphate + NAD+ | relative activity is 2% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
diglycerol + NAD+ | relative activity is 21% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
DL-alpha-glycerophosphate + NAD+ | relative activity 11%compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
DL-glyceraldehyde + NAD+ | relative rate of reduction is 14%compared to reduction of dihydroxyacetone | Klebsiella aerogenes | 3-hydroxypyruvaldehyde + NADH | - |
? | |
ethanol + NAD+ | relative activity is 1% compared to oxidation of glycerol | Klebsiella aerogenes | acetaldehyde + NADH | - |
? | |
ethylene glycol + NAD+ | relative activity is 20% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
glycerol + NAD+ | - |
Klebsiella aerogenes | dihydroxyacetone + NADH + H+ | - |
? | |
glycerol-alpha-monochlorohydrin + NAD+ | relative rate of oxidation is 26% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
hydroxy-2-propanone + NADH | relative activity is 27% compared to oxidation of glycerol | Klebsiella aerogenes | propylene glycol + NAD+ | - |
? | |
i-inositol + NAD+ | relative activity is 18% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
isopropanol + NAD+ | relative activity is 17% compared to oxidation of glycerol | Klebsiella aerogenes | acetone + NADH | - |
? | |
methylglyoxal + NADH | relative rate of reduction is 56% compared to reduction of dihydroxyacetone | Klebsiella aerogenes | lactaldehyde + NAD+ | - |
? | |
sorbitol + NAD+ | relative activity is 3% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 11 | the initial rate of reaction increases upon increasing the pH from pH 6.8 to 11.0, past pH 11.0 the initial rate falls rapidly, oxidation of glycerol | Klebsiella aerogenes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
25°C, 10 min, 30% loss of activity | Klebsiella aerogenes |
7.5 | - |
25°C, 10 min, maximal stability, 10% loss of activity | Klebsiella aerogenes |
10 | - |
25°C, 10 min, 40% loss of activity | Klebsiella aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Klebsiella aerogenes | |
NADH | - |
Klebsiella aerogenes |