Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | at 1.85 mM 135% of the activity without activator | Klebsiella aerogenes | |
2-mercaptoethanol | 1 mM, slight activation | Klebsiella aerogenes | |
8-Quinolinol | 1 mM, slight activation | Klebsiella aerogenes | |
dithioerythritol | 1 mM, slight activation | Klebsiella aerogenes | |
NH4+ | - |
Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | strongly inhibitory at 100 mM, but slight activation in lower concentration, 1 mM | Klebsiella aerogenes | |
8-Quinolinol | 1 mM, 10 mM | Klebsiella aerogenes | |
dithioerythritol | 10 mM | Klebsiella aerogenes | |
EDTA | 3 mM, 30 mM | Klebsiella aerogenes | |
iodoacetamide | strong inhibition, 10 mM | Klebsiella aerogenes | |
N-ethylmaleimide | - |
Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
74.3 | - |
glycerol | in the presence of NH4+ | Klebsiella aerogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | soluble, isolation from crude cell extract | Klebsiella aerogenes | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates | Klebsiella aerogenes | |
Rb+ | activates | Klebsiella aerogenes | |
Tl+ | activates | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Klebsiella aerogenes 1033 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | - |
Klebsiella aerogenes | dihydroxyacetone + NADH + H+ | - |
? | |
glycerol + NAD+ | - |
Klebsiella aerogenes 1033 | dihydroxyacetone + NADH + H+ | - |
? |