Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Sus scrofa | |
cysteine | slight | Sus scrofa | |
dithiothreitol | slight | Sus scrofa | |
Hg2+ | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
progesterone | - |
Sus scrofa | |
0.004 | - |
pregnenolone | - |
Sus scrofa | |
0.0086 | - |
deoxycorticosterone | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30500 | - |
gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + NADPH + H+ | Sus scrofa | - |
17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ | - |
? | |
progesterone + NADPH | Sus scrofa | - |
20beta-hydroxy-pregn-4-ene-3-one + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.004 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, 50 mM potassium phosphate buffer, pH 7.4 | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxypregnenolone + NADPH | i.e. 3beta,17alpha-dihydroxypregn-5-ene-20-one | Sus scrofa | 3beta,17alpha,20beta-trihydroxypregn-5-ene + NADP+ | - |
? | |
17alpha-hydroxyprogesterone + NADPH + H+ | - |
Sus scrofa | 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+ | - |
? | |
17alpha-hydroxyprogesterone + NADPH + H+ | - |
Sus scrofa | 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ | - |
? | |
deoxycorticosterone + NADPH | i.e. 21-hydroxypregn-4-ene-3,20-dione, 21-hydroxyprogesterone | Sus scrofa | 20,21-dihydroxypregn-4-ene-3-one + NADP+ | - |
? | |
deoxycortisol + NADPH | - |
Sus scrofa | ? | - |
? | |
pregnenolone + NADPH | i.e. 3-hydroxypregn-5-en-20-one | Sus scrofa | 3beta,20beta-Dihydroxypregn-5-ene + NADP+ | - |
? | |
progesterone + NAD(P)H | - |
Sus scrofa | 20beta-hydroxy-pregn-4-ene-3-one + NAD(P)+ | - |
? | |
progesterone + NADPH | - |
Sus scrofa | 20beta-hydroxy-pregn-4-ene-3-one + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30500, SDS-PAGE with and without 2-mercaptoethanol | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Sus scrofa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | 50% of maximal activity at 35°C, 10.4% of maximal activity at 60°C | Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
up to | Sus scrofa |
55 | - |
inactivation at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
reduction of oxosteroid | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5.5 | - |
Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Sus scrofa |