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Literature summary for 1.1.1.50 extracted from

  • Stuhlemmer, U.; Kreis, W.
    Cardenolide formation and activity of pregnane-modifying enzymes in cell suspension cultures, shoot cultures and leaves of Digitalis lanata (1996), Plant Physiol. Biochem., 34, 85-91.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ strong inhibition of oxidation of 3alpha-hydroxysteroids Digitalis lanata

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol NADPH as cofactor Digitalis lanata 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5beta-pregnan-3,20-dione + NAD(P)H Digitalis lanata 3alpha-hydroxysteroid-5beta-oxidoreductase activity 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
?

Organism

Organism UniProt Comment Textmining
Digitalis lanata
-
floxglove, NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity
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Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture low activity Digitalis lanata
-
leaf low activity in young leaves Digitalis lanata
-
shoot culture Digitalis lanata
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.56
-
substrate 5beta-dihydroprogesterone, young leaves Digitalis lanata
869.8
-
substrate 5beta-dihydroprogesterone, green shoot culture Digitalis lanata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3alpha-hydroxy-5beta-pregnan-20-one + NADP+ inhibition by Mg2+ only in oxidative direction Digitalis lanata 5beta-pregnan-3,20-dione + NADPH
-
r
5beta-pregnan-3,20-dione + NAD(P)H 3alpha-hydroxysteroid-5beta-oxidoreductase activity Digitalis lanata 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
?
5beta-pregnan-3,20-dione + NAD(P)H 3alpha-hydroxysteroid-5beta-oxidoreductase activity Digitalis lanata 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
assay at Digitalis lanata

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Digitalis lanata