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Literature summary for 1.1.1.50 extracted from

  • Adachi, O.; Toyama, H.; Matsushita, K.
    Purification and properties of 3alpha-hydroxysteroid dehydrogenase from Pseudomonas putida (1997), Biosci. Biotechnol. Biochem., 61, 131-137.
No PubMed abstract available
Search for more literature for 1.1.1.50

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pseudomonas putida

General Stability

General Stability Organism
stable in solution for a long period Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 NAD+
-
 Pseudomonas putida
0.0136
-
 deoxycholate
-
 Pseudomonas putida
0.015
-
 cholate
-
 Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Pseudomonas putida 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 non-denaturing gel electrophoresis and gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas putida belongs to the group of Y'bile acid binders, bile acid metabolizing enzymes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.6
-
 purified enzyme, substrate cholate with NAD+ Pseudomonas putida

Storage Stability

Storage Stability Organism
-80°C, lyophilized or ammonium sulfate precipitated, more than 1 year Pseudomonas putida
4°C, diluted in Tris-HCl, pH 8.0, with ß-mercaptoethanol, 20 days Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine + NAD+
-
 Pseudomonas putida 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oyl glycine + NADH
-
 ?
6-ketolithocholate + NAD+
-
 Pseudomonas putida 5beta-cholan-3,6-dione-24-oic acid + NADH
-
 r
androsterone + NAD(P)+
-
 Pseudomonas putida 5alpha-androstan-3,17-dione + NAD(P)H
-
 ?
chenodeoxycholic acid + NAD(P)+
-
 Pseudomonas putida 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 r
cholic acid + NAD(P)+
-
 Pseudomonas putida 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 ?
deoxycholic acid + NAD(P)+
-
 Pseudomonas putida 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
 ?
additional information belongs to the group of Y'bile acid binders, bile acid metabolizing enzymes Pseudomonas putida ?
-
 ?
taurocholic acid + NAD(P)+
-
 Pseudomonas putida 2[[7alpha,12alpha-dihydroxy-3,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADPH
-
 ?
ursodeoxycholate + NAD+
-
 Pseudomonas putida 7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 25000, SDS-PAGE Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 heating for 10 min, rapid heat denaturation over 50°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10 11 oxidation of cholate Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+ specific, enzyme does not use NADP+ Pseudomonas putida