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Literature summary for 1.1.1.49 extracted from

  • Adem, S.; Ciftci, M.
    Purification of rat kidney glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and glutathione reductase enzymes using 2,5-ADP Sepharose 4B affinity in a single chromatography step (2012), Protein Expr. Purif., 81, 1-4.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
2 * 68000, SDS-PAGE Rattus norvegicus
144000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + NADP+ Rattus norvegicus
-
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 531fold from kidney, co-purification with 6-phosphogluconate dehydrogenase and glutathione reductase in one chromatographic step by 2',5'-ADP affinity chromatography by using different elution buffers, method development and optimization, overview Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32
-
purified enzyme, pH and temperature not specified in the publication Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + NADP+
-
Rattus norvegicus D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 68000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
G6PD
-
Rattus norvegicus
glucose 6-phosphate dehydrogenase
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Rattus norvegicus