Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADPH | product inhibition with NADP+ or D-glucose 6-phosphate as the varying substrate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
NADP+ | 25°C, pH 8.0 | Escherichia coli | |
0.174 | - |
D-glucose 6-phosphate | 25°C, pH 8.0, cofactor: NADP+ | Escherichia coli | |
1.25 | - |
D-glucose 6-phosphate | 25°C, pH 8.0, cofactor: NAD+ | Escherichia coli | |
5.09 | - |
NAD+ | 25°C, pH 8.0 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Escherichia coli | first enzyme of the pentose phosphate pathway, one of the main sources of NADPH | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NAD+ | NADP+-specific enzyme. In presence of NAD+ 410fold reduction in the performance of the enzyme | Escherichia coli | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | first enzyme of the pentose phosphate pathway, one of the main sources of NADPH | Escherichia coli | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | NADP+-specific enzyme. In presence of NAD+ 410fold reduction in the performance of the enzyme. Modeling of the sensitivity of reduced cofactor production by G6PDH as a function of the redox ratios of NAD/NADH (rRNAD) and NADP/NADPH (rRNADP). NADPH production sharply increases within the range of thermodynamically feasible values of rRNADP, but NADH production remains low within the range feasible for rRNAD | Escherichia coli | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6PDH | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
174 | - |
NADP+ | 25°C, pH 8.0 | Escherichia coli | |
288 | - |
NAD+ | 25°C, pH 8.0 | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NADP+-specific enzyme. In presence of NAD+ 410fold reduction in the performance of the enzyme | Escherichia coli | |
NADP+ | NADP+-specific enzyme. In presence of NAD+ 410fold reduction in the performance of the enzyme. Modeling of the sensitivity of reduced cofactor production by G6PDH as a function of the redox ratios of NAD/NADH (rRNAD) and NADP/NADPH (rRNADP). NADPH production sharply increases within the range of thermodynamically feasible values of rRNADP, but NADH production remains low within the range feasible for rRNAD | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
NADPH | 25°C, pH 8.0, competitive inhibition constant | Escherichia coli | |
2.09 | - |
NADPH | 25°C, pH 8.0, non-competitive inhibition constant | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
56 | - |
NAD+ | 25°C, pH 8.0 | Escherichia coli | |
23200 | - |
NADP+ | 25°C, pH 8.0 | Escherichia coli |