Cloned (Comment) | Organism |
---|---|
wild-type and clinical mutants expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G488S | clinical mutant G6PDFukaya, mutation in the vicinity of the structural NADP+ site, elevated Kd values of the structural NADP+, is denatured by guanidinium hydrochloride and refolded by rapid dilution in the presence of L-Arg, NADP+ and dithiothreitol at 25°C, displays decreased thermostability and high susceptibility to chymotrypsin digestion as compared to the wild-type | Homo sapiens |
G488V | clinical mutant G6PDCampinas, mutation in the vicinity of the structural NADP+ site, elevated Kd values of the structural NADP+, is denatured by guanidinium hydrochloride and refolded by rapid dilution in the presence of L-Arg, NADP+ and dithiothreitol at 25°C, displays decreased thermostability and high susceptibility to chymotrypsin digestion as compared to the wild-type | Homo sapiens |
R393G | clinical mutant G6PDWisconsin, mutation in the vicinity of the structural NADP+ site, elevated Kd values of the structural NADP+, is denatured by guanidinium hydrochloride and refolded by rapid dilution in the presence of L-Arg, NADP+ and dithiothreitol at 25°C, displays thermostability as the wild-type and low susceptibility to chymotrypsin digestion | Homo sapiens |
R393H | clinical mutant G6PDNashville, mutation in the vicinity of the structural NADP+ site, elevated Kd values of the structural NADP+, is denatured by guanidinium hydrochloride and refolded by rapid dilution in the presence of L-Arg, NADP+ and dithiothreitol at 25°C, displays decreased thermostability and high susceptibility to chymotrypsin digestion as compared to the wild-type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chymotrypsin | - |
Homo sapiens | |
guanidinium hydrochloride | denatures | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0107 | - |
NADP+ | clinical mutant G488V | Homo sapiens | |
0.0118 | - |
NADP+ | clinical mutant G488S | Homo sapiens | |
0.0491 | - |
D-glucose 6-phosphate | clinical mutant G488S | Homo sapiens | |
0.0538 | - |
D-glucose 6-phosphate | clinical mutant G488V | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and clinical mutants purified to homogeneity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Homo sapiens | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6PD | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
212 | - |
D-glucose 6-phosphate | clinical mutant G488V | Homo sapiens | |
254 | - |
D-glucose 6-phosphate | clinical mutant G488S | Homo sapiens |