Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli KP3998 cells | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
GlcDH-I, gel filtration | Priestia megaterium |
150000 | - |
GlcDH-II, gel filtration | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Priestia megaterium | P39482 | - |
- |
Priestia megaterium | P40288 | - |
- |
Priestia megaterium IAM 1030 | - |
- |
- |
Priestia megaterium IAM 1030 | P39482 | - |
- |
Priestia megaterium IWG3 | P40288 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose CL-6B column chromatography | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + NAD+ | - |
Priestia megaterium | ? | - |
? | |
2-deoxy-D-glucose + NAD+ | - |
Priestia megaterium IAM 1030 | ? | - |
? | |
2-deoxy-D-glucose + NAD+ | - |
Priestia megaterium IWG3 | ? | - |
? | |
D-fructose + NAD+ | weak substrate | Priestia megaterium | ? | - |
? | |
D-fructose + NAD+ | weak substrate | Priestia megaterium IAM 1030 | ? | - |
? | |
D-fructose + NAD+ | weak substrate | Priestia megaterium IWG3 | ? | - |
? | |
D-galactose + NAD+ | weak substrate | Priestia megaterium | ? | - |
? | |
D-galactose + NAD+ | weak substrate | Priestia megaterium IAM 1030 | ? | - |
? | |
D-galactose + NAD+ | weak substrate | Priestia megaterium IWG3 | ? | - |
? | |
D-glucosamine + NAD+ | - |
Priestia megaterium | ? | - |
? | |
D-glucose + NAD+ | 100% activity | Priestia megaterium | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | 100% activity | Priestia megaterium IAM 1030 | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | 100% activity | Priestia megaterium IWG3 | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-mannose + NAD+ | weak substrate | Priestia megaterium | ? | - |
? | |
D-xylose + NAD+ | weak substrate | Priestia megaterium | ? | - |
? | |
additional information | no activity with N-acetyl-D-glucosamine, D-glucose-6-phosphate, D-arabinose, D-ribose, and myo-inositol | Priestia megaterium | ? | - |
? | |
additional information | no activity with N-acetyl-D-glucosamine, D-glucose-6-phosphate, D-arabinose, D-ribose, and myo-inositol | Priestia megaterium IAM 1030 | ? | - |
? | |
additional information | no activity with N-acetyl-D-glucosamine, D-glucose-6-phosphate, D-arabinose, D-ribose, and myo-inositol | Priestia megaterium IWG3 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlcDH-I | isozyme is unstable throughout pH range 4-10 | Priestia megaterium |
GlcDH-II | isozyme is stable in the alkaline pH region | Priestia megaterium |
GlcDH-IWG3 | isozyme is stable in the acidic pH region | Priestia megaterium |
glucose dehydrogenase | - |
Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
GlcDH-I1 is the most resistant isozyme against heat inactivation at 50°C (pH 6.5) | Priestia megaterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
190 | - |
D-glucose | isozyme GlcDH-I | Priestia megaterium | |
190 | - |
D-glucose | isozyme GlcDH-II | Priestia megaterium | |
390 | - |
D-glucose | isozyme GlcDH-IWG3 | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Priestia megaterium |