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Literature summary for 1.1.1.47 extracted from

  • Milburn, C.C.; Lamble, H.J.; Theodossis, A.; Bull, S.D.; Hough, D.W.; Danson, M.J.; Taylor, G.L.
    The structural basis of substrate promiscuity in glucose dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus (2006), J. Biol. Chem., 281, 14796-14804.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion using a mother liquor of 12% (v/v) polyethylene glycol 4000, 0.1 M MES (pH 5.8), and 3% (v/v) propan-2-ol Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
T41A reduced activity Saccharolobus solfataricus
T41V reduced activity Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
D-xylose wild type enzyme, NADP+ as cofactor Saccharolobus solfataricus
0.25
-
D-xylose wild type enzyme, NAD+ as cofactor Saccharolobus solfataricus
0.44
-
beta-D-galactose wild type enzyme, NADP+ as cofactor Saccharolobus solfataricus
0.57
-
beta-D-galactose wild type enzyme, NAD+ as cofactor Saccharolobus solfataricus
1.3
-
beta-D-glucose wild type enzyme, NADP+ as cofactor Saccharolobus solfataricus
1.5
-
beta-D-glucose wild type enzyme, NAD+ as cofactor Saccharolobus solfataricus
20.4
-
D-xylose mutant enzyme T41A, NADP+ as cofactor Saccharolobus solfataricus
24.8
-
beta-D-glucose mutant enzyme T41A, NAD+ as cofactor Saccharolobus solfataricus
29.2
-
D-xylose mutant enzyme T41A, NAD+ as cofactor Saccharolobus solfataricus
33.3
-
beta-D-glucose mutant enzyme T41A, NADP+ as cofactor Saccharolobus solfataricus
59
-
beta-D-glucose mutant enzyme T41V, NADP+ as cofactor Saccharolobus solfataricus
65.8
-
D-xylose mutant enzyme T41V, NADP+ as cofactor Saccharolobus solfataricus
72.5
-
beta-D-glucose mutant enzyme T41V, NAD+ as cofactor Saccharolobus solfataricus
76.3
-
D-xylose mutant enzyme T41V, NAD+ as cofactor Saccharolobus solfataricus
109
-
beta-D-galactose mutant enzyme T41A, NADP+ as cofactor Saccharolobus solfataricus
118
-
beta-D-galactose mutant enzyme T41A, NAD+ as cofactor Saccharolobus solfataricus
175
-
beta-D-galactose mutant enzyme T41V, NADP+ as cofactor Saccharolobus solfataricus
204
-
beta-D-galactose mutant enzyme T41V, NAD+ as cofactor Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000 Saccharolobus solfataricus
160000
-
-
Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus O93715
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration on Superdex 75 26/60, anion exchange chromatography, and affinity chromatography using Reactive Red-500 dye affinity medium and an NaCl gradient of 0 to 1.5 M Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-galactose + NADP+
-
Saccharolobus solfataricus D-galactono-1,5-lactone + NADPH
-
?
beta-D-glucose + NAD(P)+
-
Saccharolobus solfataricus D-glucono-1,5-lactone + NAD(P)H + H+
-
?
D-xylose + NAD(P)+
-
Saccharolobus solfataricus D-xylono-1,5-lactone + NAD(P)H + H+
-
?
L-arabinose + NAD(P)+
-
Saccharolobus solfataricus ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 40000 Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
GDH
-
Saccharolobus solfataricus
glucose dehydrogenase
-
Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
beta-D-glucose mutant enzyme T41V, NADP+ as cofactor Saccharolobus solfataricus
6
-
beta-D-glucose mutant enzyme T41V, NAD+ as cofactor Saccharolobus solfataricus
7
-
D-xylose mutant enzyme T41V, NADP+ as cofactor Saccharolobus solfataricus
7
-
beta-D-galactose mutant enzyme T41V, NADP+ as cofactor Saccharolobus solfataricus
8
-
beta-D-galactose mutant enzyme T41V, NAD+ as cofactor Saccharolobus solfataricus
12
-
D-xylose mutant enzyme T41V, NAD+ as cofactor Saccharolobus solfataricus
14
-
beta-D-glucose mutant enzyme T41A, NADP+ as cofactor Saccharolobus solfataricus
22
-
D-xylose mutant enzyme T41A, NADP+ as cofactor Saccharolobus solfataricus
22
-
beta-D-galactose mutant enzyme T41A, NADP+ as cofactor Saccharolobus solfataricus
37
-
beta-D-galactose wild type enzyme, NADP+ as cofactor Saccharolobus solfataricus
39
-
beta-D-glucose mutant enzyme T41A, NAD+ as cofactor Saccharolobus solfataricus
44
-
D-xylose wild type enzyme, NADP+ as cofactor Saccharolobus solfataricus
48
-
beta-D-glucose wild type enzyme, NADP+ as cofactor Saccharolobus solfataricus
56
-
beta-D-galactose mutant enzyme T41A, NAD+ as cofactor Saccharolobus solfataricus
61
-
D-xylose wild type enzyme, NAD+ as cofactor Saccharolobus solfataricus
61
-
beta-D-galactose wild type enzyme, NAD+ as cofactor Saccharolobus solfataricus
75
-
beta-D-glucose wild type enzyme, NAD+ as cofactor Saccharolobus solfataricus
81
-
D-xylose mutant enzyme T41A, NAD+ as cofactor Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharolobus solfataricus
NADP+
-
Saccharolobus solfataricus