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Literature summary for 1.1.1.47 extracted from

  • Lamble, H.J.; Heyer, N.I.; Bull, S.D.; Hough, D.W.; Danson, M.J.
    Metabolic pathway promiscuity in the archaeon Sulfolobus solfataricus revealed by studies on glucose dehydrogenase and 2-keto-3-deoxygluconate aldolase (2003), J. Biol. Chem., 278, 34066-34072.
    View publication on PubMed
Search for more literature for 1.1.1.47

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 60% inhibition at 10 mM Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 NADP+ with beta-D-galactose, pH 7.5, 70°C Saccharolobus solfataricus
0.57
-
 NAD+ with beta-D-galactose, pH 7.5, 70°C Saccharolobus solfataricus
1.3
-
 NADP+ with beta-D-glucose, pH 7.5, 70°C Saccharolobus solfataricus
1.5
-
 NAD+ with beta-D-glucose, pH 7.5, 70°C Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by 0.1 mM CaCl2 and MgCl2 Saccharolobus solfataricus
Zn2+ enzyme contains the catalytic zinc binding residues Cys39 and His66, no increase in activity by addition of 0.1 mM ZnCl2 Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
-
 Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucose + NAD(P)+ Saccharolobus solfataricus first step of the non-phosphorylative Entner-Doudoroff pathway D-glucono-1,5-lactone + NAD(P)H + H+
-
 ir

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus O93715
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by gel filtration, recombinant enzyme from Escherichia coli by heat denaturation, and affinity gel chromatography Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + NAD(P)+ low activity Saccharolobus solfataricus ?
-
 ?
6-deoxy-D-glucose + NAD(P)+
-
 Saccharolobus solfataricus ?
-
 ?
beta-D-glucose + NAD(P)+
-
 Saccharolobus solfataricus D-glucono-1,5-lactone + NAD(P)H + H+
-
 ir
beta-D-glucose + NAD(P)+ first step of the non-phosphorylative Entner-Doudoroff pathway Saccharolobus solfataricus D-glucono-1,5-lactone + NAD(P)H + H+
-
 ir
D-fucose + NAD(P)+
-
 Saccharolobus solfataricus ?
-
 ?
D-galactose + NAD(P)+ equally active compared to beta-D-glucose Saccharolobus solfataricus D-galactono-1,5-lactone + NAD(P)H
-
 ?
D-glucosamine + NAD(P)+ low activity Saccharolobus solfataricus ?
-
 ?
D-idose + NAD(P)+ low activity Saccharolobus solfataricus ?
-
 ?
D-lyxose + NAD(P)+ low activity Saccharolobus solfataricus ?
-
 ?
D-xylose + NAD(P)+
-
 Saccharolobus solfataricus ?
-
 ?
L-arabinose + NAD(P)+
-
 Saccharolobus solfataricus ?
-
 ?
additional information D-ribose, maltose, lactose and sucrose are poor substrates, no activity with D-allose, D-arabinose, and D-mannose Saccharolobus solfataricus ?
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
glucose dehydrogenase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37.4
-
 NADP+ with beta-D-galactose, pH 7.5, 70°C Saccharolobus solfataricus
47.7
-
 NADP+ with beta-D-glucose, pH 7.5, 70°C Saccharolobus solfataricus
61.3
-
 NAD+ with beta-D-galactose, pH 7.5, 70°C Saccharolobus solfataricus
74.9
-
 NAD+ with beta-D-glucose, pH 7.5, 70°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
additional information dual cofactor specificity due to presence of Asn215, His217, and the GXGXXA motif Saccharolobus solfataricus
NAD+
-
 Saccharolobus solfataricus
NADP+
-
 Saccharolobus solfataricus