Crystallization (Comment) | Organism |
---|---|
precipitate is dissolved in deionized water and ammonium sulfate added to 23% saturation | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Sus scrofa | |
Fe2+ | - |
Sus scrofa | |
PCMB | - |
Sus scrofa | |
Zn2+ | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
NAD+ | pH 9.9, 30°C | Sus scrofa | |
70 | - |
beta-D-glucose | pH 9.9, 30°C | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, acetic acid pH 5.5, Sephadex G200, G150, crystallization | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.27 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + NAD+ | - |
Sus scrofa | D-glucono-1,5-lactone + NADH + H+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Sus scrofa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 48 | 15°C: about 35% of maximal activity, 48°C: 60% of maximal activity | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.9 | - |
- |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | about 45% of maximal activity at pH 8.0 and pH 11.0 | Sus scrofa |