Cloned (Comment) | Organism |
---|---|
E96K mutant enzyme | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
E96A | mutation increases thermostability at pH 6.5 | Priestia megaterium |
E96G | mutation increases thermostability at pH 6.5 | Priestia megaterium |
E96K | mutation increases thermostability by about 15°C at pH 6.5 | Priestia megaterium |
Q252L | mutation increases thermostability at pH 6.5 | Priestia megaterium |
Y253C | mutation increases thermostability at pH 6.5 | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0051 | - |
NADP+ | E96K mutant | Priestia megaterium | |
0.0076 | - |
NADP+ | E96G mutant | Priestia megaterium | |
0.0093 | - |
NADP+ | E96A mutant | Priestia megaterium | |
0.018 | - |
NAD+ | E96K mutant | Priestia megaterium | |
0.026 | - |
NADP+ | Q252L mutant | Priestia megaterium | |
0.027 | - |
NADP+ | wild type | Priestia megaterium | |
0.037 | - |
NADP+ | Y253C mutant | Priestia megaterium | |
0.17 | - |
NAD+ | E96G mutant | Priestia megaterium | |
0.19 | - |
NAD+ | Q252L mutant | Priestia megaterium | |
0.25 | - |
NAD+ | Y253C mutant | Priestia megaterium | |
0.31 | - |
NAD+ | E96A mutant | Priestia megaterium | |
0.37 | - |
NAD+ | wild type | Priestia megaterium | |
2.7 | - |
beta-D-glucose | E96K mutant, coenzyme NAD+ | Priestia megaterium | |
4 | - |
beta-D-glucose | E96K mutant, coenzyme NADP+ | Priestia megaterium | |
8.7 | - |
beta-D-glucose | E96A mutant, coenzyme NAD+ | Priestia megaterium | |
9 | - |
beta-D-glucose | Q252L mutant, coenzyme NAD+ | Priestia megaterium | |
9.5 | - |
beta-D-glucose | wild type, coenzyme NAD+ | Priestia megaterium | |
10 | - |
beta-D-glucose | Q252L mutant, coenzyme NADP+ | Priestia megaterium | |
11 | - |
beta-D-glucose | wild type, coenzyme NADP+ | Priestia megaterium | |
13 | - |
beta-D-glucose | E96A mutant, coenzyme NADP+ | Priestia megaterium | |
16 | - |
beta-D-glucose | E96G mutant, coenzyme NAD+ | Priestia megaterium | |
18 | - |
beta-D-glucose | E96G mutant, coenzyme NADP+ | Priestia megaterium | |
55 | - |
beta-D-glucose | Y253C mutant, coenzyme NAD+ | Priestia megaterium | |
85 | - |
beta-D-glucose | Y253C mutant, coenzyme NADP+ | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + NAD+ | - |
Priestia megaterium | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucose + NADP+ | - |
Priestia megaterium | D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 6.5, 20 min, wild-type enzyme is stable | Priestia megaterium |
50 | - |
pH 6.5, 20 min, complete inactivation of wild-type enzyme, mutant enzyme E96K, E96G, E96A, Q252L and Y253C are stable | Priestia megaterium |
55 | - |
pH 6.5, 20 min, about 65% loss of acticity of mutant enzyme Y253C, about 20% loss of activity of mutant enzyme Y252L, mutant enzyme E96K, E96G and E96A are stable | Priestia megaterium |
60 | - |
pH 6.5, 20 min, complete loss of activity of enzyme Y253C about 95% loss of activity of mutant enzyme Y252L, about 30% loss of activity of mutant enzyme E96G, mutant enzymes E96K and E95A are stable | Priestia megaterium |
65 | - |
pH 6.5, 20 min, complete loss of activity of mutant enzyme Q252L, about 50% loss of activity of mutant enzyme E96K, about 10% loss of activity of mutant enzyme E96A | Priestia megaterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
beta-D-glucose | E96K mutant, coenzyme NADP+ | Priestia megaterium | |
18 | - |
NADP+ | E96K mutant | Priestia megaterium | |
23 | - |
NAD+ | E96K mutant | Priestia megaterium | |
23 | - |
beta-D-glucose | E96K mutant, coenzyme NAD+ | Priestia megaterium | |
92 | - |
beta-D-glucose | E96G mutant, coenzyme NADP+ | Priestia megaterium | |
92 | - |
NADP+ | E96G mutant | Priestia megaterium | |
97 | - |
NAD+ | E96A mutant | Priestia megaterium | |
97 | - |
beta-D-glucose | E96A mutant, coenzyme NAD+ | Priestia megaterium | |
98 | - |
beta-D-glucose | E96A mutant, coenzyme NADP+ | Priestia megaterium | |
98 | - |
NADP+ | E96A mutant | Priestia megaterium | |
114 | - |
NAD+ | E96G mutant | Priestia megaterium | |
114 | - |
beta-D-glucose | E96G mutant, coenzyme NAD+ | Priestia megaterium | |
186 | - |
beta-D-glucose | Y253C mutant, coenzyme NADP+ | Priestia megaterium | |
186 | - |
NADP+ | Y253C mutant | Priestia megaterium | |
200 | - |
NAD+ | Y253C mutant | Priestia megaterium | |
200 | - |
beta-D-glucose | Y253C mutant, coenzyme NAD+ | Priestia megaterium | |
260 | - |
NADP+ | wild type | Priestia megaterium | |
260 | - |
beta-D-glucose | wild type, coenzyme NADP+ | Priestia megaterium | |
300 | - |
beta-D-glucose | Q252L mutant, coenzyme NADP+ | Priestia megaterium | |
300 | - |
NADP+ | Q252L mutant | Priestia megaterium | |
390 | - |
NAD+ | wild type, coenzyme NAD+ | Priestia megaterium | |
390 | - |
beta-D-glucose | wild type, coenzyme NAD+ | Priestia megaterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | 30°C, 20 min, mutant enzyme Q252L is stable | Priestia megaterium |
4 | 10 | 30°C, 20 min, mutant enzymes E96K and E95A, less than 20% loss of activity | Priestia megaterium |
5 | - |
30°C, 20 min, about 55% loss of activity of mutant enzyme Y253C, about 35% loss of activity of mutant enzyme E96G and wild-type enzyme | Priestia megaterium |
6 | 7 | 30°C, 20 min, wild-type and mutant enzyme Y253C are stable | Priestia megaterium |
8 | - |
30°C, 20 min, about 50% loss of activity of mutant enzyme Y253C, about 80% loss of wild-type enzyme, about 30% loss of activity of mutant enzyme E96G and E96, about 15% loss of activity of mutant enzyme E96K | Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Priestia megaterium | |
NADP+ | - |
Priestia megaterium |