Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | in mM-concentration, no activation of the reverse reaction | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory iodoacetate, ethylendiamine tetraacetate, 2,2'-bipyridine, 8-hydroxyquiniline, O-phenanthroline, diethyldithiocarbamate, thiamine pyrophosphate | Sus scrofa | |
p-chloromercuribenzoate | in both directions | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic studies | Sus scrofa | |
0.04 | - |
NAD+ | cosubstrate L-gulonate, pH 8.5 | Sus scrofa | |
0.45 | - |
beta-keto-L-gulonate | cofactor NADH, pH 6.3 | Sus scrofa | |
5.1 | - |
L-gulonate | cofactor NAD+, pH 8.5 | Sus scrofa | |
5.3 | - |
L-3-hydroxybutanoate | - |
Sus scrofa | |
5.3 | - |
L-Idonate | cofactor NAD+, pH 8.5 | Sus scrofa | |
6.4 | - |
acetoacetate | cofactor NADH, pH 6.3 | Sus scrofa | |
7.3 | - |
2,3-diketo-L-gulonate | cofactor NADH, pH 6.3 | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sus scrofa | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation or inhibition Zn2+, Cd2+, Cu2+, Fe3+, Mn2+, Ni2+, Co3+, As3+ | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-gulonic acid + NAD+ | Sus scrofa | glucuronate-xylulose cycle | 3-dehydro-L-gulonic acid + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
hog | - |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Storage Stability | Organism |
---|---|
-18°C, 0.05 M K2HPO4, several weeks, little loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-keto-L-gulonate + NADH | - |
Sus scrofa | 2,3-diketo-L-gulonate + NAD+ | - |
ir | |
D-galactonic acid + NAD+ | - |
Sus scrofa | ? | - |
? | |
D-gluconic acid + NAD+ | - |
Sus scrofa | ? | - |
? | |
D-lyxonic acid + NAD+ | - |
Sus scrofa | ? | - |
? | |
D-mannonic acid + NAD+ | - |
Sus scrofa | ? | - |
? | |
D-talonic acid + NAD+ | - |
Sus scrofa | ? | - |
? | |
D-xylonic acid + NAD+ | - |
Sus scrofa | ? | - |
? | |
L-3-hydroxybutanoate + NADH | - |
Sus scrofa | acetoacetate + NAD+ | - |
r | |
L-altronic acid + NAD+ | - |
Sus scrofa | ? | - |
? | |
L-erythronic acid + NAD+ | - |
Sus scrofa | ? | - |
? | |
L-gulonic acid + NAD+ | stereospecificity for L-configuration of the beta-carbon hydroxyl group | Sus scrofa | 3-dehydro-L-gulonic acid + NADH + H+ | - |
r | |
L-gulonic acid + NAD+ | glucuronate-xylulose cycle | Sus scrofa | 3-dehydro-L-gulonic acid + NADH + H+ | - |
r | |
L-idonic acid + NAD+ | - |
Sus scrofa | ? | - |
? | |
L-ribonic acid + NAD+ | - |
Sus scrofa | ? | - |
? | |
L-threonic acid + NAD+ | - |
Sus scrofa | ? | - |
? | |
additional information | no substrates are: L-arabonic acid, D-glucuronate, D-mannuronate, l-iduronate, 6-phospho-D-gluconate, L-serine, L-threonine, beta-D-hydroxy acids, acetoacetyl-CoA, acetoacetyl-S-pantetheine | Sus scrofa | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
reduction of 3-dehydro-L-gulonic acid | Sus scrofa |
8.5 | - |
- |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | reduction of acetoacetate | Sus scrofa |
6.2 | 10 | oxidation of L-3-hydroxybutanoate | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sus scrofa | |
NADH | - |
Sus scrofa |