Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | isoform ICD-2, increase in activity in up to 200 mM NaCl. Isoform ICD-1, no influence of NaCl. Above 200 mM, NaCl is inhibitory | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | isoform ICD-2, increase in activity in up to 200 mM NaCl. Isoform ICD-1, no influence of NaCl. Above 200 mM, NaCl is inhibitory | Mycobacterium tuberculosis | |
NADPH | isoform ICD-2, competitive inhibition | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
DL-isocitrate | isoform ICD-1, presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
0.02 | - |
DL-isocitrate | isoform ICD-2, presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
0.022 | - |
DL-isocitrate | isoform ICD-1, presence of Zn2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
0.125 | - |
NADP+ | isoform ICD-1, presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
19.6 | - |
NADP+ | isoform ICD-2, presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | both isoforms ICD-1, ICD-2, saturation at 10 mM | Mycobacterium tuberculosis | |
additional information | no acitivty of isoforms with Mn2+ or Ca2+ | Mycobacterium tuberculosis | |
Zn2+ | isoform ICD-1, can replace Mg2+, saturation at 10 mM | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer | Mycobacterium tuberculosis |
49000 | - |
4 * 49000, isoform ICD-1, SDS-PAGE. Main form is dimer for isoform ICD-1 | Mycobacterium tuberculosis |
86000 | - |
2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer | Mycobacterium tuberculosis |
86000 | - |
and dimer, 3 * 86000, SDS-PAGE and chemical crosslinking, isoform ICD-2 | Mycobacterium tuberculosis |
100000 | - |
gel filtration, main peak, isoform ICD-1 | Mycobacterium tuberculosis |
180000 | - |
gel filtration, isoform ICD-2 | Mycobacterium tuberculosis |
200000 | - |
gel filtration, isoform ICD-1 | Mycobacterium tuberculosis |
320000 | - |
gel filtration, isoform ICD-2 | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
isoforms ICD-1 and ICD-2 | - |
Renatured (Comment) | Organism |
---|---|
isoform ICD-2, renaturation of protein inactivated for 30 min at 65°C by slow cooling till 55°C | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NADP+ | - |
Mycobacterium tuberculosis | 2-oxoglutarate + CO2 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer | Mycobacterium tuberculosis |
tetramer | 4 * 49000, isoform ICD-1, SDS-PAGE. Main form is dimer for isoform ICD-1 | Mycobacterium tuberculosis |
trimer | and dimer, 3 * 86000, SDS-PAGE and chemical crosslinking, isoform ICD-2 | Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | isoform ICD-2 | Mycobacterium tuberculosis |
40 | - |
isoform ICD-1 | Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
isoform ICD-1, 40% residual activity, ICD-2, 5% residual activity | Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
30 min, isoform ICD-1, irreversible thermal inactivation, isoform ICD-2, renaturation of inactivated protein by slow cooling till 55°C | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
DL-isocitrate | isoform ICD-1, presence of Zn2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
3.8 | - |
DL-isocitrate | isoform ICD-1, presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
4 | - |
NADP+ | isoform ICD-1, presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
19.6 | - |
DL-isocitrate | isoform ICD-2, presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
37.4 | - |
NADP+ | isoform ICD-2, presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
both isoforms ICD-1, ICD-2 | Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
isoform ICD-1, 30% of maximum activity, isoform ICD-2, 10% of maximum activity | Mycobacterium tuberculosis |
9.5 | - |
isoform ICD-1, 90% of maximum activity, isoform ICD-2, less than 40% of maximum activity | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | NAD+ may not replace NADP+ | Mycobacterium tuberculosis | |
NADP+ | required by both isoforms ICD-1 and ICD-2 | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
NADPH | isoform ICD-2, pH 7.5, 25°C | Mycobacterium tuberculosis |