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Literature summary for 1.1.1.42 extracted from

  • Banerjee, S.; Nandyala, A.; Podili, R.; Katoch, V.M.; Hasnain, S.E.
    Comparison of Mycobacterium tuberculosis isocitrate dehydrogenases (ICD-1 and ICD-2) reveals differences in coenzyme affinity, oligomeric state, pH tolerance and phylogenetic affiliation (2005), BMC Biochem., 6, 20.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
NaCl isoform ICD-2, increase in activity in up to 200 mM NaCl. Isoform ICD-1, no influence of NaCl. Above 200 mM, NaCl is inhibitory Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
NaCl isoform ICD-2, increase in activity in up to 200 mM NaCl. Isoform ICD-1, no influence of NaCl. Above 200 mM, NaCl is inhibitory Mycobacterium tuberculosis
NADPH isoform ICD-2, competitive inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
DL-isocitrate isoform ICD-1, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.02
-
DL-isocitrate isoform ICD-2, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.022
-
DL-isocitrate isoform ICD-1, presence of Zn2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.125
-
NADP+ isoform ICD-1, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
19.6
-
NADP+ isoform ICD-2, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ both isoforms ICD-1, ICD-2, saturation at 10 mM Mycobacterium tuberculosis
additional information no acitivty of isoforms with Mn2+ or Ca2+ Mycobacterium tuberculosis
Zn2+ isoform ICD-1, can replace Mg2+, saturation at 10 mM Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer Mycobacterium tuberculosis
49000
-
4 * 49000, isoform ICD-1, SDS-PAGE. Main form is dimer for isoform ICD-1 Mycobacterium tuberculosis
86000
-
2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer Mycobacterium tuberculosis
86000
-
and dimer, 3 * 86000, SDS-PAGE and chemical crosslinking, isoform ICD-2 Mycobacterium tuberculosis
100000
-
gel filtration, main peak, isoform ICD-1 Mycobacterium tuberculosis
180000
-
gel filtration, isoform ICD-2 Mycobacterium tuberculosis
200000
-
gel filtration, isoform ICD-1 Mycobacterium tuberculosis
320000
-
gel filtration, isoform ICD-2 Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
isoforms ICD-1 and ICD-2
-

Renatured (Commentary)

Renatured (Comment) Organism
isoform ICD-2, renaturation of protein inactivated for 30 min at 65°C by slow cooling till 55°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-isocitrate + NADP+
-
Mycobacterium tuberculosis 2-oxoglutarate + CO2 + NADPH
-
?

Subunits

Subunits Comment Organism
dimer 2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer Mycobacterium tuberculosis
tetramer 4 * 49000, isoform ICD-1, SDS-PAGE. Main form is dimer for isoform ICD-1 Mycobacterium tuberculosis
trimer and dimer, 3 * 86000, SDS-PAGE and chemical crosslinking, isoform ICD-2 Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 40 isoform ICD-2 Mycobacterium tuberculosis
40
-
isoform ICD-1 Mycobacterium tuberculosis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
isoform ICD-1, 40% residual activity, ICD-2, 5% residual activity Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
30 min, isoform ICD-1, irreversible thermal inactivation, isoform ICD-2, renaturation of inactivated protein by slow cooling till 55°C Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
DL-isocitrate isoform ICD-1, presence of Zn2+, pH 7.5, 25°C Mycobacterium tuberculosis
3.8
-
DL-isocitrate isoform ICD-1, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
4
-
NADP+ isoform ICD-1, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
19.6
-
DL-isocitrate isoform ICD-2, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
37.4
-
NADP+ isoform ICD-2, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
both isoforms ICD-1, ICD-2 Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
isoform ICD-1, 30% of maximum activity, isoform ICD-2, 10% of maximum activity Mycobacterium tuberculosis
9.5
-
isoform ICD-1, 90% of maximum activity, isoform ICD-2, less than 40% of maximum activity Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
additional information NAD+ may not replace NADP+ Mycobacterium tuberculosis
NADP+ required by both isoforms ICD-1 and ICD-2 Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0046
-
NADPH isoform ICD-2, pH 7.5, 25°C Mycobacterium tuberculosis