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Literature summary for 1.1.1.42 extracted from
- Purification and characterization of isocitrate dehydrogenase from a hyperthermophilic archaebacterium, Caldococcus noboribetus (1997), Biochim. Biophys. Acta, 1340, 227-234.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Caldococcus noboribetus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
NADP+ | - |
Caldococcus noboribetus |  |
| 0.072 | - |
D,L-isocitrate | - |
Caldococcus noboribetus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement for divalent cations | Caldococcus noboribetus | |
| Mn2+ | absolute requirement for divalent cations | Caldococcus noboribetus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
alpha2, 2 * 48000, SDS-PAGE | Caldococcus noboribetus |
| 90000 | - |
gel filtration | Caldococcus noboribetus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | Caldococcus noboribetus | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldococcus noboribetus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Caldococcus noboribetus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 144 | - |
- |
Caldococcus noboribetus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | - |
Caldococcus noboribetus | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha2, 2 * 48000, SDS-PAGE | Caldococcus noboribetus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
nearly no activity at 40°C or lower | Caldococcus noboribetus |
| 80 | - |
no loss in activity | Caldococcus noboribetus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Caldococcus noboribetus |
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