Cloned (Comment) | Organism |
---|---|
cloned and overexpressed in Escherichia coli | Methylococcus capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0517 | - |
isocitrate | - |
Methylococcus capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36340 | - |
4 * 36340, the clasp-like domain of McIDH is a likely site for tetramerization, calculated from sequence | Methylococcus capsulatus |
145000 | - |
gel filtration | Methylococcus capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | the enzyme is NAD+-dependent | Methylococcus capsulatus | 2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 36340, the clasp-like domain of McIDH is a likely site for tetramerization, calculated from sequence | Methylococcus capsulatus |
Synonyms | Comment | Organism |
---|---|---|
McIDH | - |
Methylococcus capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | - |
Methylococcus capsulatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
apparent midpoint melting temperature | Methylococcus capsulatus |