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Literature summary for 1.1.1.41 extracted from

  • Soundar, S.; Park, J.H.; Huh, T.L.; Colman, R.F.
    Evaluation by mutagenesis of the importance of 3 arginines in alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase (2003), J. Biol. Chem., 278, 52146-52153.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP allosteric regulation, determination of mol ADP bound to an enzyme tetramer of wild-type and mutant enzymes at different starting concentrations, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the wild-type and a mutant enzyme, the latter containing the wild-type 2 subunits alpha and beta plus an additional third one, gamma, overexpression of the 3-subunit mutant and the same mutant with point mutations of Arg-residues in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
R88Q site-directed mutagenesis, residue of the alpha-subunit, inactive mutant Homo sapiens
R97Q site-directed mutagenesis, residue of the additional mutant gamma-subunit, highly reduced activity compared to the wild-type enzyme Homo sapiens
R99Q site-directed mutagenesis, residue of the beta-subunit, reduced activity compared to the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
NAD+ purified recombinant mutant gamma-R97Q, pH 7.2, 25°C Homo sapiens
0.014
-
NAD+ purified recombinant mutant beta-R99Q, pH 7.2, 25°C Homo sapiens
0.073
-
NAD+ purified recombinant wild-type enzyme, pH 7.2, 25°C Homo sapiens
0.22
-
Mn2+ purified recombinant wild-type enzyme and mutant beta-R99Q, pH 7.2, 25°C Homo sapiens
0.32
-
isocitrate purified recombinant wild-type enzyme, pH 7.2, 25°C, in presence of 1 mM ADP Homo sapiens
0.39
-
Mn2+ purified recombinant mutant gamma-R97Q, pH 7.2, 25°C Homo sapiens
0.77
-
isocitrate purified recombinant mutant beta-R99Q, pH 7.2, 25°C, in presence of 1 mM ADP Homo sapiens
0.9
-
isocitrate purified recombinant mutant beta-R99Q, pH 7.2, 25°C Homo sapiens
1.8
-
isocitrate purified recombinant mutant gamma-R97Q, pH 7.2, 25°C, in presence of 1 mM ADP Homo sapiens
2
-
isocitrate purified recombinant mutant gamma-R97Q, pH 7.2, 25°C Homo sapiens
2.2
-
isocitrate purified recombinant wild-type enzyme, pH 7.2, 25°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
315000
-
about, recombinant wild-type and mutant enzymes, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
threo-Ds-isocitrate + NAD+ Homo sapiens
-
2-oxoglutarate + CO2 + NADH
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH + H+ Arg97, and Arg99 contribute to activity, Arg88 is essential for catalysis Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
purified recombinant mutant gamma-R97Q Homo sapiens
13.5
-
purified recombinant mutant beta-R99Q Homo sapiens
21.7
-
purified recombinant wild-type enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
threo-Ds-isocitrate + NAD+
-
Homo sapiens 2-oxoglutarate + CO2 + NADH
-
?

Subunits

Subunits Comment Organism
More enzyme subunit ratio of recombinant wild-type and mutant enzymes, overview Homo sapiens

Synonyms

Synonyms Comment Organism
NAD-dependent isocitrate dehydrogenase
-
Homo sapiens
threo-DS-isocitrate:NAD+ oxidoreductase (decarboxylating)
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for Homo sapiens