Cloned (Comment) | Organism |
---|---|
gene icd, DNA and amino acid sequence determination and analysis, expression in enzyme-deficient Escherichia coli strain EB106 | Acidithiobacillus thiooxidans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic properties of the native and the recombinant enzyme are similar | Acidithiobacillus thiooxidans | |
0.18 | - |
NAD+ | pH 9.0, 30°C, native enzyme | Acidithiobacillus thiooxidans | |
6.57 | - |
NADP+ | pH 9.0, 30°C, native enzyme | Acidithiobacillus thiooxidans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Acidithiobacillus thiooxidans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Acidithiobacillus thiooxidans |
90000 | - |
gel filtration and native PAGE | Acidithiobacillus thiooxidans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | Acidithiobacillus thiooxidans | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | Acidithiobacillus thiooxidans ON107 | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus thiooxidans | Q8GAX0 | - |
- |
Acidithiobacillus thiooxidans ON107 | Q8GAX0 | - |
- |
Purification (Comment) | Organism |
---|---|
multi-step purification of the native enzyme, 520fold to homogeneity, partial purification of the recombinant enzyme from Escherichia coli | Acidithiobacillus thiooxidans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
120 | - |
purified native enzyme | Acidithiobacillus thiooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | - |
Acidithiobacillus thiooxidans | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | - |
Acidithiobacillus thiooxidans ON107 | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NADP+ | - |
Acidithiobacillus thiooxidans | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
isocitrate + NADP+ | - |
Acidithiobacillus thiooxidans ON107 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Acidithiobacillus thiooxidans |
Synonyms | Comment | Organism |
---|---|---|
ICDH | - |
Acidithiobacillus thiooxidans |
NAD+-dependent isocitrate dehydrogenase | - |
Acidithiobacillus thiooxidans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Acidithiobacillus thiooxidans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
stable up to | Acidithiobacillus thiooxidans |
70 | - |
inactivation after 30 min | Acidithiobacillus thiooxidans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.3 | - |
NADP+ | pH 9.0, 30°C, native enzyme | Acidithiobacillus thiooxidans | |
45.1 | - |
NAD+ | pH 9.0, 30°C, native enzyme | Acidithiobacillus thiooxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Acidithiobacillus thiooxidans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10.2 | - |
Acidithiobacillus thiooxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | highly preferred cofactor, 78fold higher activity than with NADP+ | Acidithiobacillus thiooxidans | |
NADP+ | 78fold lower activity than with NAD+ | Acidithiobacillus thiooxidans |