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Literature summary for 1.1.1.41 extracted from

  • Inoue, H.; Tamura, T.; Ehara, N.; Nishito, A.; Nakayama, Y.; Maekawa, M.; Imada, K.; Tanaka, H.; Inagaki, K.
    Biochemical and molecular characterization of the NAD(+)-dependent isocitrate dehydrogenase from the chemolithotroph Acidithiobacillus thiooxidans (2002), FEMS Microbiol. Lett., 214, 127-132.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene icd, DNA and amino acid sequence determination and analysis, expression in enzyme-deficient Escherichia coli strain EB106 Acidithiobacillus thiooxidans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic properties of the native and the recombinant enzyme are similar Acidithiobacillus thiooxidans
0.18
-
NAD+ pH 9.0, 30°C, native enzyme Acidithiobacillus thiooxidans
6.57
-
NADP+ pH 9.0, 30°C, native enzyme Acidithiobacillus thiooxidans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Acidithiobacillus thiooxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
2 * 45000, SDS-PAGE Acidithiobacillus thiooxidans
90000
-
gel filtration and native PAGE Acidithiobacillus thiooxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NAD+ Acidithiobacillus thiooxidans
-
2-oxoglutarate + CO2 + NADH + H+
-
?
isocitrate + NAD+ Acidithiobacillus thiooxidans ON107
-
2-oxoglutarate + CO2 + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Acidithiobacillus thiooxidans Q8GAX0
-
-
Acidithiobacillus thiooxidans ON107 Q8GAX0
-
-

Purification (Commentary)

Purification (Comment) Organism
multi-step purification of the native enzyme, 520fold to homogeneity, partial purification of the recombinant enzyme from Escherichia coli Acidithiobacillus thiooxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
120
-
purified native enzyme Acidithiobacillus thiooxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NAD+
-
Acidithiobacillus thiooxidans 2-oxoglutarate + CO2 + NADH + H+
-
?
isocitrate + NAD+
-
Acidithiobacillus thiooxidans ON107 2-oxoglutarate + CO2 + NADH + H+
-
?
isocitrate + NADP+
-
Acidithiobacillus thiooxidans 2-oxoglutarate + CO2 + NADPH + H+
-
r
isocitrate + NADP+
-
Acidithiobacillus thiooxidans ON107 2-oxoglutarate + CO2 + NADPH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 45000, SDS-PAGE Acidithiobacillus thiooxidans

Synonyms

Synonyms Comment Organism
ICDH
-
Acidithiobacillus thiooxidans
NAD+-dependent isocitrate dehydrogenase
-
Acidithiobacillus thiooxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Acidithiobacillus thiooxidans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
stable up to Acidithiobacillus thiooxidans
70
-
inactivation after 30 min Acidithiobacillus thiooxidans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.3
-
NADP+ pH 9.0, 30°C, native enzyme Acidithiobacillus thiooxidans
45.1
-
NAD+ pH 9.0, 30°C, native enzyme Acidithiobacillus thiooxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Acidithiobacillus thiooxidans

pH Range

pH Minimum pH Maximum Comment Organism
6 10.2
-
Acidithiobacillus thiooxidans

Cofactor

Cofactor Comment Organism Structure
NAD+ highly preferred cofactor, 78fold higher activity than with NADP+ Acidithiobacillus thiooxidans
NADP+ 78fold lower activity than with NAD+ Acidithiobacillus thiooxidans