Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Haemophilus influenzae |
Protein Variants | Comment | Organism |
---|---|---|
K386A | mutant impaired for reduction of D-ribulose. Equimolar mixtures of mutants K386A and R18A are efficient for bifunctional catalysis | Haemophilus influenzae |
R18A | mutant impaired for cytidylyl transfer. Equimolar mixtures of mutants K386A and R18A are efficient for bifunctional catalysis | Haemophilus influenzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
NADPH | mutant R18A, pH 7.2, 25°C | Haemophilus influenzae | |
0.038 | - |
(2R,3R)-2,3-dihydroxy-4-oxobutyl-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
0.039 | - |
NADPH | mutant K386A, pH 7.2, 25°C | Haemophilus influenzae | |
0.044 | - |
NADPH | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
0.076 | - |
D-ribulose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
0.19 | - |
D-ribulose 5-phosphate | mutant K386A, pH 7.2, 25°C | Haemophilus influenzae | |
0.27 | - |
D-ribulose 5-phosphate | mutant R18A, pH 7.2, 25°C | Haemophilus influenzae | |
0.6 | - |
NADH | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1 | - |
D-ribulose | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
2.2 | - |
D-ribose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
3 | - |
D-arabinose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
3.5 | - |
D-xylulose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | Q48230 | additionally catalyzes the reaction of ribitol-5-phosphate cytidylyltransferase, EC 2.7.7.40 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose 5-phosphate + NADPH + H+ | - |
Haemophilus influenzae | ? + NADP+ | - |
? | |
D-erythrose 4-phosphate + NADPH + H+ | - |
Haemophilus influenzae | ? + NADP+ | - |
? | |
D-ribose 5-phosphate + NADPH + H+ | - |
Haemophilus influenzae | ? + NADP+ | - |
? | |
D-ribulose + NADPH + H+ | - |
Haemophilus influenzae | D-ribitol + NADP+ | - |
? | |
D-ribulose 5-phosphate + NADPH + H+ | - |
Haemophilus influenzae | D-ribitol 5-phosphate + NADP+ | - |
? | |
D-xylulose 5-phosphate + NADH + H+ | - |
Haemophilus influenzae | ? + NAD+ | - |
? | |
additional information | the synthase first catalyzes the reduction of D-ribulose 5-phosphate followed by cytidylyl transfer to D-ribitol 5-phosphate, with a 650fold kinetic preference for cytidylyl transfer to D-ribitol 5-phosphate over D-ribulose 5-phosphate | Haemophilus influenzae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bcs1 | - |
Haemophilus influenzae |
bifunctional ribulose 5-phosphate reductase/CDP-ribitol pyrophosphorylase | - |
Haemophilus influenzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
D-ribulose 5-phosphate | mutant K386A, pH 7.2, 25°C | Haemophilus influenzae | |
0.5 | - |
(2R,3R)-2,3-dihydroxy-4-oxobutyl-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
2.3 | - |
D-arabinose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
3.6 | - |
D-xylulose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
3.6 | - |
D-ribulose | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
4.1 | - |
D-ribose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
6.8 | - |
D-ribulose 5-phosphate | mutant R18A, pH 7.2, 25°C | Haemophilus influenzae | |
22 | - |
NADPH | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
22 | - |
D-ribulose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00008 | - |
D-arabinose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
0.0001 | - |
D-xylulose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
0.0002 | - |
D-ribose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
0.0002 | - |
D-ribulose 5-phosphate | mutant K386A, pH 7.2, 25°C | Haemophilus influenzae | |
0.0003 | - |
D-ribulose | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
0.0008 | - |
NADPH | mutant K386A, pH 7.2, 25°C | Haemophilus influenzae | |
0.0013 | - |
(2R,3R)-2,3-dihydroxy-4-oxobutyl-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
0.0025 | - |
D-ribulose 5-phosphate | mutant R18A, pH 7.2, 25°C | Haemophilus influenzae | |
0.029 | - |
D-ribulose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
0.04 | - |
NADPH | mutant R18A, pH 7.2, 25°C | Haemophilus influenzae | |
0.05 | - |
NADPH | wild-type, pH 7.2, 25°C | Haemophilus influenzae |