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Literature summary for 1.1.1.405 extracted from

  • Zolli, M.; Kobric, D.J.; Brown, E.D.
    Reduction precedes cytidylyl transfer without substrate channeling in distinct active sites of the bifunctional CDP-ribitol synthase from Haemophilus influenzae (2001), Biochemistry, 40, 5041-5048.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Haemophilus influenzae

Protein Variants

Protein Variants Comment Organism
K386A mutant impaired for reduction of D-ribulose. Equimolar mixtures of mutants K386A and R18A are efficient for bifunctional catalysis Haemophilus influenzae
R18A mutant impaired for cytidylyl transfer. Equimolar mixtures of mutants K386A and R18A are efficient for bifunctional catalysis Haemophilus influenzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
NADPH mutant R18A, pH 7.2, 25°C Haemophilus influenzae
0.038
-
(2R,3R)-2,3-dihydroxy-4-oxobutyl-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
0.039
-
NADPH mutant K386A, pH 7.2, 25°C Haemophilus influenzae
0.044
-
NADPH wild-type, pH 7.2, 25°C Haemophilus influenzae
0.076
-
D-ribulose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
0.19
-
D-ribulose 5-phosphate mutant K386A, pH 7.2, 25°C Haemophilus influenzae
0.27
-
D-ribulose 5-phosphate mutant R18A, pH 7.2, 25°C Haemophilus influenzae
0.6
-
NADH wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1
-
D-ribulose wild-type, pH 7.2, 25°C Haemophilus influenzae
2.2
-
D-ribose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
3
-
D-arabinose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
3.5
-
D-xylulose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae Q48230 additionally catalyzes the reaction of ribitol-5-phosphate cytidylyltransferase, EC 2.7.7.40
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinose 5-phosphate + NADPH + H+
-
Haemophilus influenzae ? + NADP+
-
?
D-erythrose 4-phosphate + NADPH + H+
-
Haemophilus influenzae ? + NADP+
-
?
D-ribose 5-phosphate + NADPH + H+
-
Haemophilus influenzae ? + NADP+
-
?
D-ribulose + NADPH + H+
-
Haemophilus influenzae D-ribitol + NADP+
-
?
D-ribulose 5-phosphate + NADPH + H+
-
Haemophilus influenzae D-ribitol 5-phosphate + NADP+
-
?
D-xylulose 5-phosphate + NADH + H+
-
Haemophilus influenzae ? + NAD+
-
?
additional information the synthase first catalyzes the reduction of D-ribulose 5-phosphate followed by cytidylyl transfer to D-ribitol 5-phosphate, with a 650fold kinetic preference for cytidylyl transfer to D-ribitol 5-phosphate over D-ribulose 5-phosphate Haemophilus influenzae ?
-
?

Synonyms

Synonyms Comment Organism
Bcs1
-
Haemophilus influenzae
bifunctional ribulose 5-phosphate reductase/CDP-ribitol pyrophosphorylase
-
Haemophilus influenzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.32
-
D-ribulose 5-phosphate mutant K386A, pH 7.2, 25°C Haemophilus influenzae
0.5
-
(2R,3R)-2,3-dihydroxy-4-oxobutyl-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
2.3
-
D-arabinose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
3.6
-
D-xylulose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
3.6
-
D-ribulose wild-type, pH 7.2, 25°C Haemophilus influenzae
4.1
-
D-ribose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
6.8
-
D-ribulose 5-phosphate mutant R18A, pH 7.2, 25°C Haemophilus influenzae
22
-
NADPH wild-type, pH 7.2, 25°C Haemophilus influenzae
22
-
D-ribulose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00008
-
D-arabinose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
0.0001
-
D-xylulose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
0.0002
-
D-ribose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
0.0002
-
D-ribulose 5-phosphate mutant K386A, pH 7.2, 25°C Haemophilus influenzae
0.0003
-
D-ribulose wild-type, pH 7.2, 25°C Haemophilus influenzae
0.0008
-
NADPH mutant K386A, pH 7.2, 25°C Haemophilus influenzae
0.0013
-
(2R,3R)-2,3-dihydroxy-4-oxobutyl-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
0.0025
-
D-ribulose 5-phosphate mutant R18A, pH 7.2, 25°C Haemophilus influenzae
0.029
-
D-ribulose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
0.04
-
NADPH mutant R18A, pH 7.2, 25°C Haemophilus influenzae
0.05
-
NADPH wild-type, pH 7.2, 25°C Haemophilus influenzae