Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CTP | marked stimulation, presence of 50 mM CTP decreases the Km value for ribulose 5-phosphate from 400 to 50 microM | Haemophilus influenzae | |
additional information | not stimulated by UTP, ATP, GTP, ADP, or dCTP | Haemophilus influenzae |
Cloned (Comment) | Organism |
---|---|
expression in ERscherichia coli | Haemophilus influenzae |
General Stability | Organism |
---|---|
23°C, at 0.3 mg/ml in the presence of 20 mM HEPES, pH 7.1, 1 mM DTT, and 0.5 mg of BSA/ml, ribitol 5-phosphate dehydrogenase activity decreases to about 50% of the initial activity after 2 h | Haemophilus influenzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: 0.5 mM ribitol 5-phosphate or 0.5 mM NADP+ | Haemophilus influenzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | pH 7.1, 30°C | Haemophilus influenzae | |
0.05 | - |
D-ribitol 5-phosphate | presence of CTP, pH 7.1, 30°C | Haemophilus influenzae | |
0.4 | - |
D-ribitol 5-phosphate | pH 7.1, 30°C | Haemophilus influenzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | Q48154 | additionally catalyzes reaction of ribitol-5-phosphate cytidylyltransferase, EC 2.7.7.40 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.73 | - |
substrate D-ribulose 5-phosphate, pH 8.5, 30°C | Haemophilus influenzae |
2.87 | - |
substrate D-ribulose 5-phosphate, presence of CTP, pH 8.5, 30°C | Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribitol 5-phosphate + NADP+ | - |
Haemophilus influenzae | D-ribulose 5-phosphate + NADPH + H+ | - |
r | |
D-ribulose 5-phosphate + NADPH + H+ | - |
Haemophilus influenzae | D-ribitol 5-phosphate + NADP+ | - |
r | |
additional information | the dehydrogenase activity of Acs1 is specific for ribulose 5-phosphate and NADPH in one direction and for ribitol 5-phosphate and NADP+ in the other direction. The activity is about twofold higher with ribulose 5-phosphate as the substrate than with ribose 5-phosphate. No substrate: xylulose 5-phosphate | Haemophilus influenzae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Haemophilus influenzae |
Synonyms | Comment | Organism |
---|---|---|
ACS1 | - |
Haemophilus influenzae |
bifunctional ribulose 5-phosphate reductase/CDP-ribitol pyrophosphorylase | - |
Haemophilus influenzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.4 | broad | Haemophilus influenzae |
General Information | Comment | Organism |
---|---|---|
physiological function | Acs1 encodes a bifunctional enzyme that converts ribulose 5-phosphate into ribitol 5-phosphate and further into CDP-ribitol, the activated precursor form for incorporation of ribitol 5-phosphate into the Haemophilus influenzae type a capsular polysaccharide | Haemophilus influenzae |