KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0115 | - |
NADH | pH 7.6, 22°C | Sphingobium chlorophenolicum | |
0.0176 | - |
cytochrome c | pH 7.6, 22°C | Sphingobium chlorophenolicum | |
0.0383 | - |
2,6-dichloroindophenol | pH 7.6, 22°C | Sphingobium chlorophenolicum | |
1.21 | - |
NADPH | pH 7.6, 22°C | Sphingobium chlorophenolicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
- |
Sphingobium chlorophenolicum |
100000 | - |
gel fitlration | Sphingobium chlorophenolicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+ | Sphingobium chlorophenolicum | - |
2,3,5,6-tetrachlorohydroquinone + NAD+ | - |
? | |
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+ | Sphingobium chlorophenolicum RA2 | - |
2,3,5,6-tetrachlorohydroquinone + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium chlorophenolicum | M9WIZ7 | - |
- |
Sphingobium chlorophenolicum | M9WLE1 | - |
- |
Sphingobium chlorophenolicum RA2 | M9WIZ7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, golden-brown enzyme with a spectrum consistent with the presence of both a flavin and an iron-sulfur cluster | Sphingobium chlorophenolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+ | - |
Sphingobium chlorophenolicum | 2,3,5,6-tetrachlorohydroquinone + NAD+ | - |
? | |
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+ | - |
Sphingobium chlorophenolicum RA2 | 2,3,5,6-tetrachlorohydroquinone + NAD+ | - |
? | |
2,6-dichloroindophenol + NADH + H+ | - |
Sphingobium chlorophenolicum | reduced 2,6-dichloroindophenol + NAD+ | - |
? | |
2,6-dichloroindophenol + NADH + H+ | - |
Sphingobium chlorophenolicum RA2 | reduced 2,6-dichloroindophenol + NAD+ | - |
? | |
cytochrome c + NADPH + H+ | - |
Sphingobium chlorophenolicum | reduced cytochrome c + NADP+ | - |
? | |
cytochrome c + NADPH + H+ | - |
Sphingobium chlorophenolicum RA2 | reduced cytochrome c + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 36000, calculated | Sphingobium chlorophenolicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
NADPH | pH 7.6, 22°C | Sphingobium chlorophenolicum | |
9.1 | - |
cytochrome c | pH 7.6, 22°C | Sphingobium chlorophenolicum | |
14.1 | - |
2,6-dichloroindophenol | pH 7.6, 22°C | Sphingobium chlorophenolicum | |
20.1 | - |
NADH | pH 7.6, 22°C | Sphingobium chlorophenolicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Sphingobium chlorophenolicum | |
NADH | - |
Sphingobium chlorophenolicum | |
NADPH | poor activity with NADPH | Sphingobium chlorophenolicum |
General Information | Comment | Organism |
---|---|---|
physiological function | tetrachlorohydroquinone reductase PcpD catalyzes the reduction of tetrachlorohydroquinone to tetrachlorohydroquinone. In the presence of PcpD, tetrachlorohydroquinone formed by pentachlorophenol hydroxylase PcpB is sequestered until it is reduced to the less toxic tetrachlorohydroquinone, protecting the bacterium from the toxic effects of tetrachlorohydroquinone and maintaining flux through the pathway. Deletion of pcpD results in increased sensitivity to phenols that are degraded via a benzoquinone intermediate | Sphingobium chlorophenolicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
NADPH | pH 7.6, 22°C | Sphingobium chlorophenolicum | |
368 | - |
2,6-dichloroindophenol | pH 7.6, 22°C | Sphingobium chlorophenolicum | |
510 | - |
cytochrome c | pH 7.6, 22°C | Sphingobium chlorophenolicum | |
1740 | - |
NADH | pH 7.6, 22°C | Sphingobium chlorophenolicum | |
12000 | - |
2,3,5,6-tetrachloro-1,4-benzoquinone | pH 7.6, 22°C | Sphingobium chlorophenolicum |