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Literature summary for 1.1.1.40 extracted from

  • Murugan, S.; Hung, H.C.
    Biophysical characterization of the dimer and tetramer interface interactions of the human cytosolic malic enzyme (2012), PLoS ONE, 7, e50143.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
H142A site-directed mutagenesis, a dimeric tetramer interface mutant Homo sapiens
H142A/D568A site-directed mutagenesis, a dimeric tetramer interface mutant. The mutant dimer completely dissociates into monomers after a 2.5 M urea treatment Homo sapiens
H51A/D139A site-directed mutagenesis, a dimeric dimer interface mutant Homo sapiens
H51A/D90A site-directed mutagenesis, a dimeric dimer interface mutant. The mutant dimer completely dissociates into monomers after a 1.5 M urea treatment Homo sapiens
W572A site-directed mutagenesis, a dimeric tetramer interface mutant Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ does not influence the thermal stability of the enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Subunits

Subunits Comment Organism
More the enzyme is first dissociated from a tetramer to dimers before the 2 M urea treatment, and the dimers then dissociated into monomers before the 2.5 M urea treatment Homo sapiens
tetramer c-NADP-ME has a dimer-dimer quaternary structure in which the dimer interface associates more tightly than the tetramer interface Homo sapiens

Synonyms

Synonyms Comment Organism
c-NADP-ME
-
Homo sapiens
cytosolic malic enzyme
-
Homo sapiens
cytosolic NADP+-dependent malic enzyme
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
Mg2+ does not influence the thermal stability of the enzyme Homo sapiens
52 64 thermal denaturation of c-NADP-ME wild-type and the interface mutants, overview Homo sapiens