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Literature summary for 1.1.1.40 extracted from

  • Yu, Q.; Liu, J.; Wang, Z.; Nai, J.; Lü, M.; Zhou, X.; Cheng, Y.
    Characterization of the NADP-malic enzymes in the woody plant Populus trichocarpa (2013), Mol. Biol. Rep., 40, 1385-1396.
    View publication on PubMed
Search for more literature for 1.1.1.40

Activating Compound

Activating Compound Comment Organism Structure
acetyl-CoA PtNADP-ME1, PtNADP-ME3, and PtNADP-ME4 are activated Populus trichocarpa
D-glucose 6-phosphate activates PtNADP-ME3 and PtNADP-ME4 Populus trichocarpa

Cloned(Commentary)

Cloned (Comment) Organism
five NADP-ME genes PtNADP-ME1, PtNADP-ME2, PtNADP-ME3, PtNADP-ME4, and PtNADPME5, DNA and amino acid sequence determination and analysis, phylogenetic analysis, individual expression of GST-tagged isozymes in Escherichia coli Populus trichocarpa

Inhibitors

Inhibitors Comment Organism Structure
Citric acid inhibits all PtNADP-ME activities significantly Populus trichocarpa
CoA activities of PtNADP-ME1, PtNADP-ME2 and PtNADPME4 proteins are inhibited Populus trichocarpa
D-fructose PtNADP-ME1 Populus trichocarpa
oxaloacetic acid inhibits all PtNADP-ME activities significantly Populus trichocarpa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information among the five isoforms, PtNADP-ME5 has the highest affinities for NADP while PtNADP-ME2 exhibits the lowest affinities toward both malate and NADP+ Populus trichocarpa
0.03
-
 NADP+ recombinant isozyme PtNADP-ME5, pH 7.5, 30°C Populus trichocarpa
0.036
-
 NADP+ recombinant isozyme PtNADP-ME1, pH 7.5, 30°C Populus trichocarpa
0.075
-
 NADP+ recombinant isozyme PtNADP-ME4, pH 7.5, 30°C Populus trichocarpa
0.1
-
 NADP+ recombinant isozyme PtNADP-ME3, pH 7.5, 30°C Populus trichocarpa
0.12
-
 NADP+ recombinant isozyme PtNADP-ME2, pH 7.5, 30°C Populus trichocarpa
1
-
 (S)-malate recombinant isozyme PtNADP-ME1, pH 7.5, 30°C Populus trichocarpa
1.1
-
 (S)-malate recombinant isozyme PtNADP-ME5, pH 7.5, 30°C Populus trichocarpa
1.4
-
 (S)-malate recombinant isozyme PtNADP-ME4, pH 7.5, 30°C Populus trichocarpa
1.5
-
 (S)-malate recombinant isozyme PtNADP-ME3, pH 7.5, 30°C Populus trichocarpa
2.9
-
 (S)-malate recombinant isozyme PtNADP-ME2, pH 7.5, 30°C Populus trichocarpa

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast putative plastidic isozymes PtNADP-ME4 and PtNADP-ME5 Populus trichocarpa 9507
-
cytosol isozymes PtNADP-ME2 and PtNADPME3 Populus trichocarpa 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Populus trichocarpa
-
 pyruvate + CO2 + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Populus trichocarpa
-
 five NADP-ME genes, PtNADP-ME1, PtNADP-ME2, PtNADP-ME3, PtNADP-ME4, and PtNADPME5
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozymes PtNADP-ME1, PtNADP-ME2, PtNADP-ME3, PtNADP-ME4, and PtNADPME5 from Escherichia coli by glutathione affinity chromatography Populus trichocarpa

Source Tissue

Source Tissue Comment Organism Textmining
additional information transcription levels of PtNADP-ME1 in lignified stems and roots are clearly higher than in other tissues, and PtNADP-ME2, PtNADP-ME3, PtNADP-ME4 and PtNADPME5 are broadly expressed in various tissues Populus trichocarpa
-
root
-
 Populus trichocarpa
-
stem lighified Populus trichocarpa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
 Populus trichocarpa pyruvate + CO2 + NADPH
-
 ?

Subunits

Subunits Comment Organism
oligomer the cytosolic PtNADP-ME2 aggregates as octamers and hexadecamers while the plastidic PtNADP-ME4 resembles hexamers and octamers. Different PtNADP-ME family members present different oligomeric states in vitro Populus trichocarpa

Synonyms

Synonyms Comment Organism
NADP-malic enzyme
-
 Populus trichocarpa
NADP-ME
-
 Populus trichocarpa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Populus trichocarpa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 NADP+ recombinant isozyme PtNADP-ME1, pH 7.5, 30°C Populus trichocarpa
0.9
-
 (S)-malate recombinant isozyme PtNADP-ME1, pH 7.5, 30°C Populus trichocarpa
12.6
-
 (S)-malate recombinant isozyme PtNADP-ME2, pH 7.5, 30°C Populus trichocarpa
13.2
-
 NADP+ recombinant isozyme PtNADP-ME2, pH 7.5, 30°C Populus trichocarpa
13.6
-
 (S)-malate recombinant isozyme PtNADP-ME3, pH 7.5, 30°C Populus trichocarpa
14.2
-
 NADP+ recombinant isozyme PtNADP-ME3, pH 7.5, 30°C Populus trichocarpa
15.7
-
 (S)-malate recombinant isozyme PtNADP-ME4, pH 7.5, 30°C Populus trichocarpa
15.9
-
 NADP+ recombinant isozyme PtNADP-ME4, pH 7.5, 30°C Populus trichocarpa
19.1
-
 (S)-malate recombinant isozyme PtNADP-ME5, pH 7.5, 30°C Populus trichocarpa
19.5
-
 NADP+ recombinant isozyme PtNADP-ME5, pH 7.5, 30°C Populus trichocarpa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Populus trichocarpa

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Populus trichocarpa

Expression

Organism Comment Expression
Populus trichocarpa significant decrease of PtNADP-ME2 and PtNADP-ME3 in mRNA levels, but only a slight change in other PtNADP-ME genes, during the dark down
Populus trichocarpa NaCl salt stress upregulates the transcripts of putative plastidic isozymes PtNADP-ME4 and PtNADP-ME5 significantly. Expression of PtNADP-ME2 and PtNADP-ME3 increases during the course of leaf wounding, PtNADP-ME3 transcript levels peak at 6 h under NaCl and mannitol stresses. Expression of PtNADP-ME1 gene is induced slightly by mannitol and PEG osmotic stresses, but not by salt stress up

General Information

General Information Comment Organism
evolution the five PtNADP-ME isoforms cluster in a phylogenetic tree constructed with the whole set of plant NADP-ME sequences, classification into four groups. PtNADP-ME2 and PtNADPME3 cluster with the cytosolic dicot NADP-ME group (group II), while PtNADP-ME4 and PtNADP-ME5 are included in the plastidic dicot NADP-ME group (group III). The group IV comprises both monocot and dicot enzymes, including PtNADP-ME1. Neither of the PtNADP-ME isoforms is included in the monocot NADPMEs (group I) Populus trichocarpa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.9
-
 (S)-malate recombinant isozyme PtNADP-ME1, pH 7.5, 30°C Populus trichocarpa
4.3
-
 (S)-malate recombinant isozyme PtNADP-ME2, pH 7.5, 30°C Populus trichocarpa
9.1
-
 (S)-malate recombinant isozyme PtNADP-ME3, pH 7.5, 30°C Populus trichocarpa
11
-
 (S)-malate recombinant isozyme PtNADP-ME4, pH 7.5, 30°C Populus trichocarpa
22
-
 NADP+ recombinant isozyme PtNADP-ME1, pH 7.5, 30°C Populus trichocarpa
110
-
 NADP+ recombinant isozyme PtNADP-ME2, pH 7.5, 30°C Populus trichocarpa
140
-
 NADP+ recombinant isozyme PtNADP-ME3, pH 7.5, 30°C Populus trichocarpa
170
-
 (S)-malate recombinant isozyme PtNADP-ME5, pH 7.5, 30°C Populus trichocarpa
210
-
 NADP+ recombinant isozyme PtNADP-ME4, pH 7.5, 30°C Populus trichocarpa
650
-
 NADP+ recombinant isozyme PtNADP-ME5, pH 7.5, 30°C Populus trichocarpa