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Literature summary for 1.1.1.40 extracted from

  • Pongratz, R.L.; Kibbey, R.G.; Cline, G.W.
    Investigating the roles of mitochondrial and cytosolic malic enzyme in insulin secretion (2009), Methods Enzymol., 457, 425-450.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.40

Cloned(Commentary)

Cloned (Comment) Organism
ME1 and ME3, quantitative real time PCR expression analysis Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information siRNA knockout of ME1 in INS-1 832/13 beta-cells, siRNA knockdown and isotopic labeling strategies, method optimization, overview Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic malic enzyme ME1 Rattus norvegicus 5829
-
mitochondrion mitochondrial malic enzyme ME3 Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Rattus norvegicus
NH4+ activates Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Rattus norvegicus
-
 pyruvate + NADPH + H+ + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
INS-1 832/13 cell insulinoma cells, high expression level of isozyme ME1 Rattus norvegicus
-
pancreatic islet
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
 Rattus norvegicus pyruvate + NADPH + H+ + CO2
-
 ?

Synonyms

Synonyms Comment Organism
cytosolic malic enzyme
-
 Rattus norvegicus
cytosolic NADP+-dependent isoform
-
 Rattus norvegicus
malic enzyme 1
-
 Rattus norvegicus
malic enzyme 3
-
 Rattus norvegicus
ME1
-
 Rattus norvegicus
ME3
-
 Rattus norvegicus
NADP+-dependent malic enzyme 3
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Rattus norvegicus

General Information

General Information Comment Organism
metabolism the citrate-malate-pyruvate cycle serves to regenerate NAD+ and maintain glycolytic flux. Pyruvate cycles all lead to the exchange of reducing equivalents from mitochondrial NADH to cytosolic NADPH. Malic enzyme is integral to the coupling of metabolism with insulin secretion Rattus norvegicus
physiological function malate that is exported from the mitochondria to the cytosol is regenerated to pyruvate by cytosolic malic enzyme for cycling back to the mitochondria. Cytosolic malic enzyme, together with ATP citrate lyase and malate dehydrogenase, is also central to recycling of citrate back to pyruvate. Cytosolic malic enzyme in the beta-cell supports the concept that the mechanisms linking metabolism with insulin secretion may include a beta-cell pyruvate-malate cycle. siRNA knockdown and isotopic labeling strategies to evaluate the role of cytosolic and mitochondrial isozymes of malic enzyme in facilitating malate-pyruvate cycling in the context of fuel-stimulated insulin secretion, overview Rattus norvegicus