Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Mortierella alpina | the only enzyme that can provide NADPH for fatty acid biosynthesis in oleaginous microorganisms, isozyme E, which arises from isoform D, is associated with lipid accumulation, overview | pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mortierella alpina | - |
an oleaginous fungus, strain CBS 696.70, at least seven isozymes A-G | - |
Purification (Comment) | Organism |
---|---|
separation of isozymes in native PAGE | Mortierella alpina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | only two isozymes present in the fermenter-grown or bottle-grown cultures | Mortierella alpina | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mortierella alpina |
Storage Stability | Organism |
---|---|
during the storage of the whole cells at -20°C, isozyme E is gradually converted to isozyme G | Mortierella alpina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Mortierella alpina | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | the only enzyme that can provide NADPH for fatty acid biosynthesis in oleaginous microorganisms, isozyme E, which arises from isoform D, is associated with lipid accumulation, overview | Mortierella alpina | pyruvate + CO2 + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
malic enzyme | - |
Mortierella alpina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mortierella alpina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mortierella alpina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mortierella alpina | |
NADPH | - |
Mortierella alpina |