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Literature summary for 1.1.1.40 extracted from

  • Zhang, Y.; Ratledge, C.
    Multiple isoforms of malic enzyme in the oleaginous fungus, Mortierella alpina (2008), Mycol. Res., 112, 725-730.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Mortierella alpina the only enzyme that can provide NADPH for fatty acid biosynthesis in oleaginous microorganisms, isozyme E, which arises from isoform D, is associated with lipid accumulation, overview pyruvate + CO2 + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Mortierella alpina
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an oleaginous fungus, strain CBS 696.70, at least seven isozymes A-G
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Purification (Commentary)

Purification (Comment) Organism
separation of isozymes in native PAGE Mortierella alpina

Source Tissue

Source Tissue Comment Organism Textmining
cell culture only two isozymes present in the fermenter-grown or bottle-grown cultures Mortierella alpina
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Mortierella alpina

Storage Stability

Storage Stability Organism
during the storage of the whole cells at -20°C, isozyme E is gradually converted to isozyme G Mortierella alpina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Mortierella alpina pyruvate + CO2 + NADPH
-
r
(S)-malate + NADP+ the only enzyme that can provide NADPH for fatty acid biosynthesis in oleaginous microorganisms, isozyme E, which arises from isoform D, is associated with lipid accumulation, overview Mortierella alpina pyruvate + CO2 + NADPH
-
r

Synonyms

Synonyms Comment Organism
malic enzyme
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Mortierella alpina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mortierella alpina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mortierella alpina

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Mortierella alpina
NADPH
-
Mortierella alpina