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Literature summary for 1.1.1.40 extracted from
- Identification of domains involved in tetramerization and malate inhibition of maize C4-NADP-malic enzyme (2007), J. Biol. Chem., 282, 6053-6060.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged chimeric NADP-ME mutants in Escherichia coli strain BL21(DE3) | Zea mays |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of chimerical NADP-ME sequences obtained from C4 and non-C4 isozymes | Zea mays |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | competitive | Zea mays |  |
| citrate | competitive | Zea mays | |
| malate | inhibition at high concentrations at pH 7.0, but not at pH 8.0 | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of recombinant chimeric mutant enzymes, overview | Zea mays | |
| 0.04 | - |
NADP+ | pH 7.0, wild-type C4-NADP-ME isozyme | Zea mays | |
| 0.04 | - |
(S)-malate | pH 7.0, wild-type C4-NADP-ME isozyme | Zea mays | |
| 0.51 | - |
NADP+ | pH 7.0, wild-type non-C4-NADP-ME isozyme | Zea mays | |
| 0.51 | - |
(S)-malate | pH 7.0, wild-type non-C4-NADP-ME isozyme | Zea mays | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Zea mays | 9507 | - |
| cytosol | - |
Zea mays | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Zea mays | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | Zea mays | - |
pyruvate + CO2 + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
C4 NADP-ME and non-C4 NADP-ME isozymes, i.e. L and R isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged chimeric NADP-ME mutants from Escherichia coli strain BL21(DE3) by two different steps of affinity chromatography to homogeneity | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Zea mays | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Zea mays | pyruvate + CO2 + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000-66000, recombinant chimeric enzymes, SDS-PAGE | Zea mays |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C4 photosynthetic NADP-malic enzyme | - |
Zea mays |
| C4-NADP-malic enzyme | - |
Zea mays |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 104.4 | - |
(S)-malate | pH 7.0, wild-type C4-NADP-ME isozyme | Zea mays | |
| 151.7 | - |
(S)-malate | pH 7.0, wild-type non-C4-NADP-ME isozyme | Zea mays | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Zea mays | |
| NADPH | - |
Zea mays | |
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Release 2023.1 (January 2023)
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