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Literature summary for 1.1.1.40 extracted from

  • Doubnerova, V.; Jiraskova, A.; Janoskova, M.; Mueller, K.; Batkova, P.; Synkova, H.; Cerovska, N.; Ryslava, H.
    The activity and isoforms of NADP-malic enzyme in Nicotiana benthamiana plants under biotic stress (2007), Gen. Physiol. Biophys., 26, 281-289.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Nicotiana benthamiana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Nicotiana benthamiana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
4 * 62000, tissue specific isozymes, SDS-PAGE, 4 * 70000, tissue specific isozymes, SDS-PAGE Nicotiana benthamiana
70000
-
monomeric flower isozyme, native PAGE Nicotiana benthamiana
140000
-
dimeric flower isozyme, native PAGE Nicotiana benthamiana
248000
-
tetrameric isozymes from leaves, stem and roots, native PAGE, overview Nicotiana benthamiana
280000
-
tetrameric isozymes from flower, leaves, and stem, native PAGE, overview Nicotiana benthamiana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Nicotiana benthamiana
-
pyruvate + CO2 + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Nicotiana benthamiana
-
wild-type plants and transgenic plants, expressing potyviral helper component protease HC-pro or Potato virus Y strain NTN
-

Source Tissue

Source Tissue Comment Organism Textmining
epidermal cell
-
Nicotiana benthamiana
-
flower isozyme F1 and F2 Nicotiana benthamiana
-
leaf isozymes L2, L3 and L4 in older leaves, isozyme L1/R in younger leaves and roots, cell saround the midrib, as well as stomatal, epidermal, and mesophyll cells Nicotiana benthamiana
-
mesophyll
-
Nicotiana benthamiana
-
additional information tissue-specific isozyme patterns, overview Nicotiana benthamiana
-
phloem
-
Nicotiana benthamiana
-
root isozyme L1/R in younger leaves and roots Nicotiana benthamiana
-
stem isozymes S1 and S2 Nicotiana benthamiana
-
stoma
-
Nicotiana benthamiana
-
xylem parenchymal cell vessel Nicotiana benthamiana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the enzyme activity is similar in Nicotiana benthamiana wild-type plants and transgenic plants, expressing potyviral helper component protease HC-pro or Potato virus Y strain NTN, overview Nicotiana benthamiana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Nicotiana benthamiana pyruvate + CO2 + NADPH
-
r
additional information the isozyme pattern is similar in Nicotiana benthamiana wild-type plants and transgenic plants, expressing potyviral helper component protease HC-pro or Potato virus Y strain NTN, overview Nicotiana benthamiana ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 70000, SDS-PAGE Nicotiana benthamiana
monomer 1 * 70000, SDS-PAGE Nicotiana benthamiana
tetramer 4 * 62000, tissue specific isozymes, SDS-PAGE, 4 * 70000, tissue specific isozymes, SDS-PAGE Nicotiana benthamiana

Synonyms

Synonyms Comment Organism
NADP-malic enzyme
-
Nicotiana benthamiana
NADP-ME
-
Nicotiana benthamiana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Nicotiana benthamiana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Nicotiana benthamiana

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Nicotiana benthamiana
NADPH
-
Nicotiana benthamiana