KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
NADP+ | 60°C, pH 8.0 | Saccharolobus solfataricus | |
0.018 | - |
(S)-malate | 60°C, pH 8.0, presence of Mn2+ | Saccharolobus solfataricus | |
0.065 | - |
(S)-malate | 60°C, pH 8.0, presence of Mg2+ | Saccharolobus solfataricus | |
0.25 | - |
NAD+ | 60°C, pH 8.0 | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | may partly replace for Mn2+ | Saccharolobus solfataricus | |
Mn2+ | most effective divalent cation | Saccharolobus solfataricus | |
additional information | absolute requirement for a divalent cation. Ca2+ is barely effective | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
2 * 49000, SDS-PAGE | Saccharolobus solfataricus |
105000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
enzyme additionally catalyzes decarboxylation of oxalacetate | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.6 | - |
60°C, pH 8.0 | Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
25°C, pH 8.0, stable for at least one month | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | NADP+ is preferred over NAD+ | Saccharolobus solfataricus | pyruvate + CO2 + NADH | - |
? | |
(S)-malate + NADP+ | NADP+ is preferred over NAD+ | Saccharolobus solfataricus | pyruvate + CO2 + NADPH | - |
? | |
additional information | enzyme additionally catalyzes decarboxylation of oxalacetate | Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 49000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for at least 24 h | Saccharolobus solfataricus |
75 | - |
stable for at least 12 h | Saccharolobus solfataricus |
80 | - |
stable for at least 7 h | Saccharolobus solfataricus |
85 | - |
stable for at least 5 h | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
376 | - |
(S)-malate | pH 8.0, 60°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Saccharolobus solfataricus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
stable for 24 h | Saccharolobus solfataricus |
8 | - |
stable for at least one month | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharolobus solfataricus | |
NADP+ | - |
Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | isoelectric focusing | - |
5.1 |