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Literature summary for 1.1.1.4 extracted from
- A 2,3-butanediol dehydrogenase from Paenibacillus polymyxa ZJ-9 for mainly producing R,R-2,3-butanediol: purification, characterization and cloning (2013), J. Basic Microbiol., 53, 733-741.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparison, subcloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21 (DE3) | Paenibacillus polymyxa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
(3R,3S)-acetoin | pH 7.0, 30°C | Paenibacillus polymyxa |  |
| 0.32 | - |
NADH | pH 7.0, 30°C | Paenibacillus polymyxa | |
| 0.84 | - |
acetoin | (S)-acetoin, pH 7.0, 30°C | Paenibacillus polymyxa | |
| 2.3 | - |
NAD+ | pH 7.0, 30°C | Paenibacillus polymyxa | |
| 2.73 | - |
meso-butane-2,3-diol | pH 7.0, 30°C | Paenibacillus polymyxa | |
| 7.67 | - |
(2R,3R)-butane-2,3-diol | pH 7.0, 30°C | Paenibacillus polymyxa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44500 | - |
2 * 44500, SDS-PAGE | Paenibacillus polymyxa |
| 90000 | - |
gel filtration | Paenibacillus polymyxa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus polymyxa | - |
isolated from soil | - |
| Paenibacillus polymyxa ZJ-9 | - |
isolated from soil | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 35fold to homogeneity by fractional ammonium sulfate precipitation, followed by two different steps of anion exchange chromatography | Paenibacillus polymyxa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Paenibacillus polymyxa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.65 | - |
purified native enzyme, pH 7.0, 30°C | Paenibacillus polymyxa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R,3R)-butane-2,3-diol + NAD+ | - |
Paenibacillus polymyxa | (3R,3S)-acetoin + NADH + H+ | - |
r | |
| (2R,3R)-butane-2,3-diol + NAD+ | - |
Paenibacillus polymyxa ZJ-9 | (3R,3S)-acetoin + NADH + H+ | - |
r | |
| meso-butane-2,3-diol + NAD+ | - |
Paenibacillus polymyxa | acetoin + NADH + H+ | - |
r | |
| meso-butane-2,3-diol + NAD+ | - |
Paenibacillus polymyxa ZJ-9 | acetoin + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 44500, SDS-PAGE | Paenibacillus polymyxa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2,3-butanediol dehydrogenase | - |
Paenibacillus polymyxa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Paenibacillus polymyxa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
oxidation reaction | Paenibacillus polymyxa |
| 8 | - |
reduction reaction | Paenibacillus polymyxa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NADP+ or NADPH as the coenzyme, respectively | Paenibacillus polymyxa | |
| NAD+ | - |
Paenibacillus polymyxa | |
| NADH | - |
Paenibacillus polymyxa | |
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