Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Arabidopsis thaliana | |
acetyl-CoA | - |
Arabidopsis thaliana | |
CoA | activation kinetics, overview | Arabidopsis thaliana | |
D-fructose 1,6-bisphosphate | - |
Arabidopsis thaliana | |
fumarate | - |
Arabidopsis thaliana | |
additional information | no activation by CoA and acetyl-CoA, poor activation by ATP and AMP | Arabidopsis thaliana | |
additional information | no activation by oxaloacetate, poor activation by ATP and AMP | Arabidopsis thaliana | |
additional information | poor activation by ATP and AMP | Arabidopsis thaliana | |
oxaloacetate | - |
Arabidopsis thaliana | |
phosphoenolpyruvate | - |
Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged NAD-ME1 and of mutants NADME1q and NAD-ME2q in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
expression of His-tagged NAD-ME2 and mutants NADME1q and NAD-ME2q in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
expression of His-tagged NAD-MEH in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of two chimeras NADME1q and NAD-ME2q by interchanging the first 176 amino residues between NAD-ME1 and -2, altered regulation in comparison to the wild-type enzymes, overview | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis and comparison of the different isozymes MAD-ME1, NAD-ME2, and NAD-MEH, and of mutants NADME1q and NAD-ME2q, overview | Arabidopsis thaliana | |
0.5 | - |
NAD+ | pH 6.4, temperature not specified in the publication, NAD-ME1 | Arabidopsis thaliana | |
0.5 | - |
NAD+ | pH 6.6, temperature not specified in the publication, NAD-ME2 | Arabidopsis thaliana | |
0.55 | - |
NAD+ | pH 6.5, temperature not specified in the publication, NAD-MEH | Arabidopsis thaliana | |
2.7 | - |
(S)-malate | pH 6.5, temperature not specified in the publication, NAD-MEH | Arabidopsis thaliana | |
3 | - |
(S)-malate | pH 6.4, temperature not specified in the publication, NAD-ME1 | Arabidopsis thaliana | |
3 | - |
(S)-malate | pH 6.6, temperature not specified in the publication, NAD-ME2 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
125000 | - |
NAD-MEH, gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Arabidopsis thaliana | - |
pyruvate + NADH + H+ + CO2 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | Q8L7K9 | isozyme NAD-ME2 | - |
Arabidopsis thaliana | Q9SIU0 | isozyme NAD-ME1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged NAD-ME1 and mutants NADME1q and NAD-ME2q from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Arabidopsis thaliana |
recombinant His-tagged NAD-ME2 and mutants NADME1q and NAD-ME2q from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filteration | Arabidopsis thaliana |
recombinant His-tagged NAD-MEH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
flower | NAD-ME1and -2 subunits show a distinct patterns of accumulation in the separate components of the floral organ | Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
sepal | NAD-MEH and NADME1 act in concert in this tissue | Arabidopsis thaliana | - |
sepal | the NAD-ME1 subunit is present at a slightly higher proportion than the NAD-ME2 subunit, and thus, NAD-MEH and NADME1 act in concert in this tissue | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Arabidopsis thaliana | pyruvate + NADH + H+ + CO2 | - |
ir | |
(S)-malate + NAD+ | very low activity in the reverse reaction in vitro | Arabidopsis thaliana | pyruvate + NADH + H+ + CO2 | - |
r | |
additional information | NAD-ME1 does not perform decarboxylation of oxaloacetate | Arabidopsis thaliana | ? | - |
? | |
additional information | NAD-ME2 does not perform decarboxylation of oxaloacetate | Arabidopsis thaliana | ? | - |
? | |
additional information | NAD-MEH does not perform decarboxylation of oxaloacetate | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | isozyme NAD-ME1 primary structure and domain analysis, overview | Arabidopsis thaliana |
More | isozyme NAD-ME2 primary structure and domain analysis, overview | Arabidopsis thaliana |
More | isozyme NAD-MEH primary structure and domain analysis, overview | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
NAD-malic enzyme | - |
Arabidopsis thaliana |
NAD-ME1 | - |
Arabidopsis thaliana |
NAD-ME2 | - |
Arabidopsis thaliana |
NAD-MEH | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31.1 | - |
NAD+ | pH 6.4, temperature not specified in the publication, NAD-ME1 | Arabidopsis thaliana | |
31.1 | - |
(S)-malate | pH 6.4, temperature not specified in the publication, NAD-ME1 | Arabidopsis thaliana | |
39 | - |
NAD+ | pH 6.5, temperature not specified in the publication, NAD-MEH | Arabidopsis thaliana | |
39 | - |
(S)-malate | pH 6.5, temperature not specified in the publication, NAD-MEH | Arabidopsis thaliana | |
44.1 | - |
NAD+ | pH 6.6, temperature not specified in the publication, NAD-ME2 | Arabidopsis thaliana | |
44.1 | - |
(S)-malate | pH 6.6, temperature not specified in the publication, NAD-ME2 | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
NAD-ME1 | Arabidopsis thaliana |
6.5 | - |
NAD-MEH | Arabidopsis thaliana |
6.6 | - |
NAD-ME2 | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
additional information | identification of specific domains of the primary structure, which are involved in the differential allosteric regulation. Different properties of NAD-ME1, -2, and -H, mitochondrial NAD-ME activity may be regulated by varying native association in vivo, rendering enzymatic entities with distinct allosteric regulation to fulfill specific roles, overview | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.3 | - |
(S)-malate | pH 6.4, temperature not specified in the publication, NAD-ME1 | Arabidopsis thaliana | |
14.2 | - |
(S)-malate | pH 6.5, temperature not specified in the publication, NAD-MEH | Arabidopsis thaliana | |
14.7 | - |
(S)-malate | pH 6.6, temperature not specified in the publication, NAD-ME2 | Arabidopsis thaliana | |
60.2 | - |
NAD+ | pH 6.4, temperature not specified in the publication, NAD-ME1 | Arabidopsis thaliana | |
67 | - |
NAD+ | pH 6.5, temperature not specified in the publication, NAD-MEH | Arabidopsis thaliana | |
88.2 | - |
NAD+ | pH 6.6, temperature not specified in the publication, NAD-ME2 | Arabidopsis thaliana |