Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 | Rhodopseudomonas palustris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | enzyme activity is allosterically regulated by acetyl-CoA, almost complete inhibition at 0.05 mM | Rhodopseudomonas palustris | |
Ca2+ | inhibits 30% at 1 mM and 60% at 10 mM | Rhodopseudomonas palustris | |
EDTA | complete inhibition at 0.1 mM | Rhodopseudomonas palustris | |
fructose 6-phosphate | competitive versus (S)-malate, 70% inhibition at 2.5 mM | Rhodopseudomonas palustris | |
Li+ | slight inhibition | Rhodopseudomonas palustris | |
Na+ | complete inhibition at 10 mM, no effect by Na+ at 1 mM | Rhodopseudomonas palustris | |
oxaloacetate | competitive versus (S)-malate, 20% inhibition at 2.5 mM | Rhodopseudomonas palustris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, overview | Rhodopseudomonas palustris | |
0.11 | - |
NAD+ | pH 7.2, 30°C, with (S)-malate | Rhodopseudomonas palustris | |
1.7 | - |
(S)-malate | pH 7.2, 30°C, with NAD+ | Rhodopseudomonas palustris | |
1.8 | - |
NADP+ | pH 7.2, 30°C, with (S)-malate | Rhodopseudomonas palustris | |
15 | - |
(S)-malate | pH 7.2, 30°C, with NADP+ | Rhodopseudomonas palustris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates 7fold at 1-10 mM | Rhodopseudomonas palustris | |
Mg2+ | activates 12fold at 1 mM and 15fold at 10 mM | Rhodopseudomonas palustris | |
Mn2+ | activates 13fold at 1 mM and 15fold at 10 mM | Rhodopseudomonas palustris | |
additional information | malic enzyme activity is markedly enhanced by mono- and divalent cations | Rhodopseudomonas palustris | |
NH4+ | activates 12fold at 1 mM and 20fold at 10 mM | Rhodopseudomonas palustris | |
Zn2+ | 5fold activation at 5 mM, only slight activation at 10 mM | Rhodopseudomonas palustris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
x * 86000 | Rhodopseudomonas palustris |
650000 | - |
- |
Rhodopseudomonas palustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Rhodopseudomonas palustris | - |
pyruvate + NADH + H+ + CO2 | - |
? | |
(S)-malate + NAD+ | Rhodopseudomonas palustris No. 7 | - |
pyruvate + NADH + H+ + CO2 | - |
? | |
(S)-malate + NADP+ | Rhodopseudomonas palustris | NADP+ shows 22% of the activity with NAD+ | pyruvate + NADPH + H+ + CO2 | - |
? | |
(S)-malate + NADP+ | Rhodopseudomonas palustris No. 7 | NADP+ shows 22% of the activity with NAD+ | pyruvate + NADPH + H+ + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodopseudomonas palustris | A4F2S6 | - |
- |
Rhodopseudomonas palustris No. 7 | A4F2S6 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 1500fold to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, adsorption chromatography, ultrafiltration, and gel filtration | Rhodopseudomonas palustris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
64 | - |
purified enzyme, pH 7.2, 30°C | Rhodopseudomonas palustris |
Storage Stability | Organism |
---|---|
-80°C, purified native enzyme, 8 months, completely stable | Rhodopseudomonas palustris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Rhodopseudomonas palustris | pyruvate + NADH + H+ + CO2 | - |
? | |
(S)-malate + NAD+ | - |
Rhodopseudomonas palustris No. 7 | pyruvate + NADH + H+ + CO2 | - |
? | |
(S)-malate + NADP+ | NADP+ shows 22% of the activity with NAD+ | Rhodopseudomonas palustris | pyruvate + NADPH + H+ + CO2 | - |
? | |
(S)-malate + NADP+ | NADP+ shows 22% of the activity with NAD+ | Rhodopseudomonas palustris No. 7 | pyruvate + NADPH + H+ + CO2 | - |
? | |
additional information | the enzyme shows 1% of the forward reaction activity in the reverse reaction and in decarboxylation oxaloacetate. D-malate and succinate are poor substrates showing 3.9% and 8.2% of the activity with (S)-malate | Rhodopseudomonas palustris | ? | - |
? | |
additional information | the enzyme shows 1% of the forward reaction activity in the reverse reaction and in decarboxylation oxaloacetate. D-malate and succinate are poor substrates showing 3.9% and 8.2% of the activity with (S)-malate | Rhodopseudomonas palustris No. 7 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 86000 | Rhodopseudomonas palustris |
Synonyms | Comment | Organism |
---|---|---|
malic enzyme | - |
Rhodopseudomonas palustris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Rhodopseudomonas palustris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
purified enzyme, stable up to, loss of activity above | Rhodopseudomonas palustris |
70 | - |
purified enzyme, almost complete inactivation | Rhodopseudomonas palustris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
480 | - |
(S)-malate | pH 7.2, 30°C, with NADP+ | Rhodopseudomonas palustris | |
520 | - |
NADP+ | pH 7.2, 30°C, with (S)-malate | Rhodopseudomonas palustris | |
720 | - |
NAD+ | pH 7.2, 30°C, with (S)-malate | Rhodopseudomonas palustris | |
770 | - |
(S)-malate | pH 7.2, 30°C, with NAD+ | Rhodopseudomonas palustris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Rhodopseudomonas palustris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
purified enzyme, most stable at | Rhodopseudomonas palustris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme uses both NAD+ and NADP+ | Rhodopseudomonas palustris | |
NAD+ | preferred cofactor | Rhodopseudomonas palustris | |
NADP+ | - |
Rhodopseudomonas palustris |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
oxaloacetate | pH 7.2, 30°C, versus (S)-malate | Rhodopseudomonas palustris | |
7.4 | - |
fructose 6-phosphate | pH 7.2, 30°C, versus (S)-malate | Rhodopseudomonas palustris |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodopseudomonas palustris | sequence calculation | - |
6.3 |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme activity is allosterically regulated by acetyl-CoA | Rhodopseudomonas palustris |
physiological function | malic enzyme plays an important role in the metabolic regulation under photoheterotrophic conditions, carbon metabolic pathway, overview | Rhodopseudomonas palustris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2900 | - |
NADP+ | pH 7.2, 30°C, with (S)-malate | Rhodopseudomonas palustris | |
3100 | - |
(S)-malate | pH 7.2, 30°C, with NAD+ | Rhodopseudomonas palustris | |
3300 | - |
(S)-malate | pH 7.2, 30°C, with NADP+ | Rhodopseudomonas palustris | |
6900 | - |
NAD+ | pH 7.2, 30°C, with (S)-malate | Rhodopseudomonas palustris |