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Literature summary for 1.1.1.39 extracted from

  • Tao, X.; Yang, Z.; Tong, L.
    Crystal structures of substrate complexes of malic enzyme and insights into the catalytic mechanism (2003), Structure, 11, 1141-1150.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
fumarate allosteric activator Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme complexed with the natural substrate malate or pyruvate, NAD+ or NADH, Mn2+, and the allosteric activator fumarate, X-ray diffraction structure determination and analysis at 2.1 A resolution, structure modeling Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD(P)+ Homo sapiens
-
pyruvate + CO2 + NAD(P)H
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(S)-malate + NAD+ = pyruvate + CO2 + NADH + H+ catalytic mechanism, malate is bound deeply in the active site, Mn2+ catalyzes the entire reaction, Lys183 is the general base for oxidation, Tyr112-Lys183 functions as the general acid-base pair to catalyze the tautomerization of the enolpyruvate product from decarboxylation to pyruvate, substrate and cofacor binding modes Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD(P)+
-
Homo sapiens pyruvate + CO2 + NAD(P)H
-
r

Synonyms

Synonyms Comment Organism
malic enzyme
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens
NADH
-
Homo sapiens
NADP+
-
Homo sapiens
NADPH
-
Homo sapiens