Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fumarate | allosteric activator | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
enzyme complexed with the natural substrate malate or pyruvate, NAD+ or NADH, Mn2+, and the allosteric activator fumarate, X-ray diffraction structure determination and analysis at 2.1 A resolution, structure modeling | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD(P)+ | Homo sapiens | - |
pyruvate + CO2 + NAD(P)H | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-malate + NAD+ = pyruvate + CO2 + NADH + H+ | catalytic mechanism, malate is bound deeply in the active site, Mn2+ catalyzes the entire reaction, Lys183 is the general base for oxidation, Tyr112-Lys183 functions as the general acid-base pair to catalyze the tautomerization of the enolpyruvate product from decarboxylation to pyruvate, substrate and cofacor binding modes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD(P)+ | - |
Homo sapiens | pyruvate + CO2 + NAD(P)H | - |
r |
Synonyms | Comment | Organism |
---|---|---|
malic enzyme | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NADH | - |
Homo sapiens | |
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |