Crystallization (Comment) | Organism |
---|---|
enzyme in binary complex with NAD+, by hanging drop vapour diffusion method, from solution containing 5 mM NAD+, 10 mM tartronate, and 20 mM MgSO4, for cryoprotection soaking overnight in 100 mM Tris-HCl, pH 7.5, with 25% PEG w/v 15 mM NAD+, 10 mM 2-mercaptoethanol, and glycerol up to a final concentration of 20% v/v, X-ray diffraction structure determination and analysis at 2.3 A resolution, structure modeling | Ascaris suum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Ascaris suum | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ascaris suum | the Ascaris suum enzyme forms complexes with the structurally similar human enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ascaris suum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
several steps | Ascaris suum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-malate + NAD+ = pyruvate + CO2 + NADH + H+ | active site structure, catalytic residues are Y126, R181, K199, D295, N343, and N479 | Ascaris suum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the Ascaris suum enzyme forms complexes with the structurally similar human enzyme | Ascaris suum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | dimer of dimers | Ascaris suum |
Synonyms | Comment | Organism |
---|---|---|
NAD-malic enzyme | - |
Ascaris suum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | binding site structure | Ascaris suum |