Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Actinomadura kijaniata |
Crystallization (Comment) | Organism |
---|---|
hanging drop method of vapor diffusion, binary and ternary complexes of KijD10 with NADP+ and dTDP-benzene, 2.0 A resolution or better | Actinomadura kijaniata |
Protein Variants | Comment | Organism |
---|---|---|
K102A | mutant protein is completely inactive | Actinomadura kijaniata |
K102E | mutant enzyme retains some activity, but its catalytic efficiency is reduced by more than 4 orders of magnitude from that for the wild-type enzyme | Actinomadura kijaniata |
K102M | mutant protein is completely inactive | Actinomadura kijaniata |
K102Q | mutant protein is completely inactive | Actinomadura kijaniata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data are determined for mutant enzymes and wild-type enzyme | Actinomadura kijaniata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+ | Actinomadura kijaniata | the enzyme is involved in the biosynthesis of dTDP-L-digitoxose | dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinomadura kijaniata | B3TMR8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Actinomadura kijaniata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+ | the enzyme is involved in the biosynthesis of dTDP-L-digitoxose | Actinomadura kijaniata | dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+ | - |
? | |
dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+ | dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose =(2R,6S)-6-hydroxy-2-methyldihydro-2H-pyran-3,4-dione | Actinomadura kijaniata | dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+ | dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose = dTDP-2,6-dideoxy-alpha-D-threo-hexopyranos-4-ulose | ? | |
dTDP-3-dehydro-6-deoxy-alpha D-galactose + NADPH + H+ | - |
Actinomadura kijaniata | dTDP-fucose + NADP+ | - |
? | |
dTDP-3-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+ | - |
Actinomadura kijaniata | dTDP-quinovose + NADP+ | - |
? | |
additional information | to functioning on its natural substrate, KijD10 can also turn over sugars that contain hydroxyl groups at the C-2 and C-4 positions. It does not discriminate with respect to the orientation of the hydroxyl group at C-4 | Actinomadura kijaniata | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KijD10 | - |
Actinomadura kijaniata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Actinomadura kijaniata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data are determined for mutant enzymes and wild-type enzyme | Actinomadura kijaniata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Actinomadura kijaniata |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of dTDP-L-digitoxose | Actinomadura kijaniata |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data are determined for mutant enzymes and wild-type enzyme | Actinomadura kijaniata |