Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli JM109 | Escherichia coli |
expression in Escherichia coli JM109 | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
NADP+ | pH 9.0, 30°C | Escherichia coli | |
3 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
7 | - |
D-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
29 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
33 | - |
L-Glycerate | pH 9.0, 30°C | Escherichia coli | |
36 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
40 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
50 | - |
D-glycerate | pH 9.0, 30°C | Escherichia coli | |
57 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
60 | - |
D-threonine | pH 9.0, 30°C | Escherichia coli | |
60 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
61 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
69 | - |
D-serine | pH 9.0, 30°C | Escherichia coli | |
71 | - |
D-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
95 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
95 | - |
L-3-hydroxybutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P39831 | - |
- |
Saccharomyces cerevisiae | Q05016 | - |
- |
Saccharomyces cerevisiae ATCC 204508 | Q05016 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
pH 9.0, 30°C | Escherichia coli |
6 | - |
pH 9.0, 30°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
D-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 31% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
D-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 1% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
D-glycerate + NADP+ | the Vmax/KM value is 20% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
D-serine + NADP+ | the Vmax/KM value is 15% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
D-serine + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
D-serine + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
D-threonine + NADP+ | the Vmax/KM value is 55% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
D-threonine + NADP+ | the Vmax/KM value is 55% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
D-threonine + NADP+ | the Vmax/KM value is 55% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
L-3-hydroxybutyrate + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
L-3-hydroxybutyrate + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
L-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 3.8% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
L-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 34% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
L-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 3.8% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
L-allo-threonine + NADP+ | highest Vmax/Km value of all substrates tested. The hydroxyl group of L-allo-threonine is oxidized by the enzymes to yield L-2-amino-3-ketobutyrate, which is spontaneously decarboxylated into aminoacetone | Escherichia coli | aminoacetone + CO2 + NADPH + H+ | - |
? | |
L-allo-threonine + NADP+ | highest Vmax/Km value of all substrates tested. The hydroxyl group of L-allo-threonine is oxidized by the enzymes to yield L-2-amino-3-oxobutyrate, which is spontaneously decarboxylated into aminoacetone | Saccharomyces cerevisiae | aminoacetone + CO2 + NADPH + H+ | - |
? | |
L-glycerate + NADP+ | the Vmax/KM value is 18% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
L-serine + NADP+ | the Vmax/KM value is 53% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
L-serine + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ydfG | - |
Escherichia coli |
YMR226c | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
30 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
when heated for 10 min in 0.1 M potassium phosphate buffer (pH 7.4) containing 0.01% 2-mercaptoethanol and 10% glycerol, the enzyme is stable at up to 40°C | Saccharomyces cerevisiae |
55 | - |
when heated for 10 min in 0.1 M potassium phosphate buffer (pH 7.4) containing 0.01% 2-mercaptoethanol and 10% glycerol, the enzyme is stable at up to 55°C | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.17 | - |
L-Glycerate | pH 9.0, 30°C | Escherichia coli | |
2.01 | - |
D-glycerate | pH 9.0, 30°C | Escherichia coli | |
3.5 | - |
D-serine | pH 9.0, 30°C | Escherichia coli | |
3.78 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
4.03 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
4.2 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
5.76 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
6.55 | - |
D-threonine | pH 9.0, 30°C | Escherichia coli | |
7.84 | - |
D-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
8.61 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
9.38 | - |
L-3-hydroxybutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
11 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
20.1 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
43.8 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
52.8 | - |
D-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
oxidation of L-serine | Escherichia coli |
8.5 | - |
oxidation of L-serine | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | most stable over the pH-range 6.5 to 10.0 | Escherichia coli |
7.5 | 10.5 | most stable over the pH-range 6.5 to 10.0 | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | no activity with NAD+ | Escherichia coli | |
NADP+ | no activity with NAD+ | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
L-Glycerate | pH 9.0, 30°C | Escherichia coli | |
0.04 | - |
D-glycerate | pH 9.0, 30°C | Escherichia coli | |
0.05 | - |
D-serine | pH 9.0, 30°C | Escherichia coli | |
0.062 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
0.067 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
0.099 | - |
L-3-hydroxybutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
0.105 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
0.109 | - |
D-threonine | pH 9.0, 30°C | Escherichia coli | |
0.11 | - |
D-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
0.116 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
0.151 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
0.199 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
0.558 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
7.5 | - |
D-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
14.6 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae |