Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | 0.5 mM, 2.7fold increase in oxaloacetate reduction activity | Escherichia coli | |
N-ethylmaleimide | 1 mM, 8.9fold increase in oxaloacetate reduction activity | Escherichia coli | |
p-chloromercuribenzoate | 0.02 mM, 2.5fold increase in oxaloacetate reduction activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | inhibition of decarboxylation activity | Escherichia coli | |
iodoacetate | inhibition of decarboxylation activity | Escherichia coli | |
N-ethylmaleimide | L-malate and tartronate strongly protect the enzyme in the presence of MnCl2 and NAD+, 80% inhibition of decarboxylation activity, 10fold increase in reduction activity | Escherichia coli | |
p-(chloromercuri)benzoate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADH | reduction of oxaloacetate | Escherichia coli | |
0.063 | - |
NAD+ | decarboxylation of L-malate | Escherichia coli | |
0.45 | - |
L-malate | decarboxylation of L-malate | Escherichia coli | |
2.1 | - |
oxaloacetate | reduction of oxaloacetate | Escherichia coli | |
4.8 | - |
oxaloacetate | decarboxylation of oxaloacetate | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Escherichia coli | - |
CO2 + pyruvate + NADH | - |
? | |
Oxaloacetate | Escherichia coli | - |
CO2 + pyruvate | - |
ir | |
oxaloacetate + NADH | Escherichia coli | - |
L-malate + NAD+ | - |
? | |
pyruvate + NADH + H+ | Escherichia coli | - |
L-lactate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.07 | - |
pyruvate reduction | Escherichia coli |
1.7 | - |
oxaloacetate reduction | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Escherichia coli | CO2 + pyruvate + NADH | - |
? | |
(S)-malate + NAD+ | - |
Escherichia coli | CO2 + pyruvate + NADH | - |
r | |
Oxaloacetate | - |
Escherichia coli | CO2 + pyruvate | - |
ir | |
oxaloacetate + NADH | - |
Escherichia coli | L-malate + NAD+ | - |
? | |
oxaloacetate + NADH | divalent metal ions are required | Escherichia coli | (S)-malate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Escherichia coli | (S)-lactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Escherichia coli | L-lactate + NAD+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
oxaloacetate reduction | Escherichia coli |
5 | - |
pyruvate reduction | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli |