Cloned (Comment) | Organism |
---|---|
gene iolW, DNA and amino acid sequence determination and analysis, the gene belongs to the Gfo/Idh/MocA family | Bacillus subtilis |
gene yulF, DNA and amino acid sequence determination and analysis, the gene belongs to the Gfo/Idh/MocA family, recombinant expression of His6-tagged YulF | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
scyllo-inositol + NADP+ | Bacillus subtilis | - |
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+ | - |
? | |
scyllo-inositol + NADP+ | Bacillus subtilis 168 | - |
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O05265 | - |
- |
Bacillus subtilis | O32223 | - |
- |
Bacillus subtilis 168 | O05265 | - |
- |
Bacillus subtilis 168 | O32223 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
scyllo-inositol + NADP+ | - |
Bacillus subtilis | 2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+ | - |
? | |
scyllo-inositol + NADP+ | recombinant His6-tagged YulF converts scyllo-inosose into SI in an NADPH-dependent manner | Bacillus subtilis | 2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+ | - |
? | |
scyllo-inositol + NADP+ | - |
Bacillus subtilis 168 | 2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+ | - |
? | |
scyllo-inositol + NADP+ | recombinant His6-tagged YulF converts scyllo-inosose into SI in an NADPH-dependent manner | Bacillus subtilis 168 | 2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
iolU | - |
Bacillus subtilis |
IolW | - |
Bacillus subtilis |
NADP+-dependent SI dehydrogenase | - |
Bacillus subtilis |
yulF | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
evolution | IolW shares a significant similarity with YulF, another NADP+-dependent SI dehydrogenase | Bacillus subtilis |
evolution | YulF shares a significant similarity with IolW, another NADP+-dependent SI dehydrogenase | Bacillus subtilis |
additional information | when yulF is overexpressed instead of iolW in the Bacillus subtilis cell factory, scyllo-inositol is produced from myo-inositol, suggesting a similar function to iolW | Bacillus subtilis |