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Literature summary for 1.1.1.37 extracted from

  • Hsieh, J.Y.; Chen, M.C.; Hung, H.C.
    Determinants of nucleotide-binding selectivity of malic enzyme (2011), PLoS ONE, 6, e25312.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.37

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol MDH1 Homo sapiens 5829
-
mitochondrion Mdh2 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Homo sapiens
-
 oxaloacetate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast dermal, activity of MDH1 is reduced in old human dermal fibroblasts Homo sapiens
-
IMR-90 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Homo sapiens oxaloacetate + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
Mdh1
-
 Homo sapiens
MDH2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens
NADH
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction knockdown of MDH1 in young human dermal fibroblasts and the IMR90 human fibroblast cell line results in the appearance of significant cellular senescence features, including senescence-associated beta-galactosidase staining, flattened and enlarged morphology, increased population doubling time, and elevated p16INK4A and p21CIP1 protein levels. The NAD/NADH ratio is decreased by 90% in MDH1 knockdown dermal fibroblasts but only by about 30% in MDH2 knockdown dermal fibroblasts Homo sapiens
physiological function MDH1 plays a critical role in the cellular senescence of human fibroblasts. MDH1 is the major regulator of the cofactor NAD, the loss of which induces cellular senescence Homo sapiens