Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, real-time quantitative RT-PCR expression analysis, recombinant ectopic expression in Solanum lycopersicum | Malus domestica |
gene MdcyMDH, DNA and amino acid sequence determination and analysis, genetic organization, expression analysis, overview. MdcyMDH gene, driven by theCaMV35S promoter, is introduced into tomato to evaluate the ex planta function of MdcyMDH ectopic expression on fruit acidity. MdcyMDH transcripts are detected in both fruits and functional leaves | Malus domestica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | MdcyMDH overexpression contributed to malate accumulation in the apple callus and tomato | Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
(S)-malate | pH 7.5, 30°C | Malus domestica | |
0.19 | - |
(S)-malate | pH 7.9, 25°C | Malus domestica | |
0.405 | - |
oxaloacetate | pH 7.8, 30°C | Malus domestica | |
1.4 | - |
NADH | pH 7.8, 30°C | Malus domestica | |
3.32 | - |
NAD+ | pH 7.5, 30°C | Malus domestica | |
3.32 | - |
NAD+ | pH 7.9, 25°C | Malus domestica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Malus domestica | 5737 | - |
cytosol | cytosolic malate dehydrogenase isozyme | Malus domestica | 5829 | - |
additional information | subcellular localization analysis, overview | Malus domestica | - |
- |
plasma membrane | - |
Malus domestica | 5886 | - |
plasma membrane | cytosolic malate dehydrogenase isozyme | Malus domestica | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Malus domestica | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | A3DSX0 | - |
- |
Malus domestica | A3DSX0 | gene MdcyMDH | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | expression level of MdcyMDH is positively correlated with MDH activity throughout fruit development, but not with malate content, especially in the ripening apple fruit | Malus domestica | - |
fruit | enzyme expression throughout fruit development | Malus domestica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Malus domestica | oxaloacetate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
cyMDH | - |
Malus domestica |
cytosolic malate dehydrogenase | - |
Malus domestica |
cytosolic NAD-dependent malate dehydrogenase | - |
Malus domestica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, malate oxidation reaction | Malus domestica |
30 | - |
assay at | Malus domestica |
30 | - |
assay at, oxaloacetate reduction reaction | Malus domestica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.54 | - |
NAD+ | pH 7.5, 30°C | Malus domestica | |
3.21 | - |
NAD+ | pH 7.9, 25°C | Malus domestica | |
8.4 | - |
(S)-malate | pH 7.9, 25°C | Malus domestica | |
9.91 | - |
(S)-malate | pH 7.5, 30°C | Malus domestica | |
609.5 | - |
NADH | pH 7.8, 30°C | Malus domestica | |
4553 | - |
oxaloacetate | pH 7.8, 30°C | Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, malate oxidation | Malus domestica |
7.8 | - |
assay at, oxaloacetate reduction | Malus domestica |
7.8 | - |
assay at, oxaloacetate reduction reaction | Malus domestica |
7.9 | - |
assay at, malate oxidation reaction | Malus domestica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Malus domestica | |
NADH | - |
Malus domestica |
General Information | Comment | Organism |
---|---|---|
physiological function | cyMDH is an enzyme crucial for malate synthesis in the cytosol. Involvement of MdcyMDH directly in malate synthesis and indirectly in malate accumulation through the regulation of genes/enzymes associated with malate degradation and transportation, gluconeogenesis and the tricarboxylic acid cycle. | Malus domestica |
physiological function | cytosolic NAD-dependent malate dehydrogenase is an enzyme crucial for malate synthesis in the cytosol | Malus domestica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.765 | - |
NAD+ | pH 7.9, 25°C | Malus domestica | |
0.77 | - |
NAD+ | pH 7.5, 30°C | Malus domestica | |
44.26 | - |
(S)-malate | pH 7.5, 30°C | Malus domestica | |
44.26 | - |
(S)-malate | pH 7.9, 25°C | Malus domestica | |
435.4 | - |
NADH | pH 7.8, 30°C | Malus domestica | |
11240 | - |
oxaloacetate | pH 7.8, 30°C | Malus domestica |