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Literature summary for 1.1.1.37 extracted from

  • Zaitseva, J.; Meneely, K.M.; Lamb, A.L.
    Structure of Escherichia coli malate dehydrogenase at 1.45 A resolution (2009), Acta Crystallogr. Sect. F, 65, 866-869.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, using 100 mM N-(2-acetamido)-iminodiacetic acid pH 6.5, 0.2 M sodium acetate, 30% (w/v) PEG MME 5000, 5% (v/v) glycerol Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32200
-
 2 * 32200, calculated from amino acid sequence Escherichia coli
64400
-
 calculated from amino acid sequence Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P61889
-
-

Purification (Commentary)

Purification (Comment) Organism
Source 30Q column chromatography and Superdex 75 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 32200, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
MDH
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Escherichia coli