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Literature summary for 1.1.1.37 extracted from
- Isolation and characterization of cytosolic malate dehydrogenase from Trichomonas vaginalis (1997), Folia Parasitol., 44, 103-108.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | therapy development for sexually transmitted disease of humans | Trichomonas vaginalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| oxaloacetate | - |
Trichomonas gallinae |  |
| oxaloacetate | - |
Trichomonas vaginalis | |
| oxaloacetate | - |
Tritrichomonas suis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0106 | - |
NADH | Tv 10-02 | Trichomonas vaginalis | |
| 0.011 | - |
NADH | - |
Trichomonas gallinae | |
| 0.013 | - |
NADH | - |
Tritrichomonas suis | |
| 0.016 | - |
oxaloacetate | - |
Trichomonas gallinae | |
| 0.0162 | - |
oxaloacetate | Tv 10-02 | Trichomonas vaginalis | |
| 0.0175 | - |
oxaloacetate | - |
Tritrichomonas suis | |
| 0.02 | - |
NADH | Tv-1961 | Trichomonas vaginalis | |
| 0.02 | - |
oxaloacetate | Tv-1961 | Trichomonas vaginalis | |
| 0.02 | - |
NADH | Tv-1973 | Trichomonas vaginalis | |
| 0.022 | - |
NAD+ | Tv-1973 | Trichomonas vaginalis | |
| 0.043 | - |
oxaloacetate | Tv-1973 | Trichomonas vaginalis | |
| 0.0699 | - |
NAD+ | Tv 10-02 | Trichomonas vaginalis | |
| 0.07 | - |
NAD+ | - |
Trichomonas gallinae | |
| 0.2 | - |
L-malate | - |
Trichomonas gallinae | |
| 1 | - |
L-malate | Tv-1961 | Trichomonas vaginalis | |
| 1.22 | - |
L-malate | Tv 10-02 | Trichomonas vaginalis | |
| 1.4 | - |
L-malate | Tv-1973 | Trichomonas vaginalis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Trichomonas vaginalis | 5829 | - |
| hydrogenosome | - |
Trichomonas vaginalis | 42566 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34500 | - |
2 * 34500, SDS-PAGE | Trichomonas vaginalis |
| 37000 | - |
6 * 37000 | Tritrichomonas suis |
| 60000 | - |
gel filtration | Trichomonas vaginalis |
| 72000 | - |
gel filtration | Trichomonas gallinae |
| 244000 | - |
gel filtration | Tritrichomonas suis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malate + NAD+ | Thermus aquaticus | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Trichomonas vaginalis | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Mycobacterium leprae | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Tritrichomonas suis | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Trichomonas gallinae | - |
oxaloacetate + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium leprae | - |
- |
- |
| Thermus aquaticus | - |
- |
- |
| Trichomonas gallinae | - |
- |
- |
| Trichomonas vaginalis | - |
strain TV 10-02, strain Tv-1961, strain Tv-1973 | - |
| Tritrichomonas suis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trichomonas vaginalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 352.7 | - |
- |
Trichomonas vaginalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | - |
Thermus aquaticus | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Trichomonas vaginalis | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Mycobacterium leprae | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Tritrichomonas suis | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Trichomonas gallinae | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Thermus aquaticus | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Trichomonas vaginalis | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Mycobacterium leprae | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Tritrichomonas suis | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Trichomonas gallinae | oxaloacetate + NADH + H+ | - |
r | |
| oxaloacetate + NADH | - |
Thermus aquaticus | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Trichomonas vaginalis | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Mycobacterium leprae | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Tritrichomonas suis | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Trichomonas gallinae | L-malate + NAD+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Thermus aquaticus |
| dimer | 2 * 34500, SDS-PAGE | Trichomonas vaginalis |
| hexamer | 6 * 37000 | Tritrichomonas suis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.3 | Tv 10-02, reduction of oxaloacetate | Trichomonas vaginalis |
| 7.8 | - |
strain Tv-1973 | Trichomonas vaginalis |
| 7.8 | 7.9 | Tv-1961, reduction of oxaloacetate | Trichomonas vaginalis |
| 8 | - |
oxaloacetate reduction | Tritrichomonas suis |
| 9 | - |
oxalacetate reduction | Trichomonas gallinae |
| 9 | - |
malate oxidation, above pH 9.0, strain Tv-1973 | Trichomonas vaginalis |
| 9.2 | 10.7 | malate oxidation, strain Tv-1961 | Trichomonas vaginalis |
| 9.5 | - |
reverse reaction from malate to oxalacetate | Trichomonas vaginalis |
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